DL-Dithiothreitol

• Catalog No.: 43815
• CAS Number: 3483-12-3
• M. F.: C4H10O2S2
• M. W.: 154.251
• Purity: ≥99.5% (RT)

SYNONYMS

• Title: DL-二硫苏糖醇
• IUPAC name: (2S,3S)-1,4-disulfanylbutane-2,3-diol
• IUPAC Traditional name: dithiothreitol
• Synonyms: threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT; 1,4-二巯基苏糖醇; 克莱兰氏试剂

DATABASE IDS

• MDL Number: MFCD00004877
• PubChem SID: 24867426
• EC Number: 222-468-7
• CAS Number: 3483-12-3
• Beilstein Number: 1719757

PROPERTIES

• Antion Traces: sulfate (SO42-): ≤50 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.5 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤100 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Grade: for molecular biology
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• Impurities: ≤0.3% 4,5-dihydroxy-1,2-dithiane
• λ: 0.1 M in H2O
• Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
• Product Line: BioUltra
• Purity: ≥99.5% (RT)
• Absorption Wavelength: λ: 260 nm Amax: 0.400
• Absorption Wavelength: λ: 280 nm Amax: 0.100
• Flash Point: 113 °C
• Flash Point: 235.4 °F
• Melting Point: 41-44 °C(lit.)
• pH: 4.0-6.5 (25 °C, 0.1 M in H2O)
• Solubility: H2O: soluble0.1 M at 20 °C, clear, colorless
• GHS Signal Word: Warning
• GHS Hazard statements: H302-H315-H319-H335
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 22-36/37/38
• RTECS: EK1610000
• Safety Statements: 26-36
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1

Description (简体中文)

Application
用于将 -SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1