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DL-Dithiothreitol_Molecular_structure_CAS_3483-12-3)
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DL-Dithiothreitol

Catalog No. 43815 Name Sigma Aldrich
CAS Number 3483-12-3 Website http://www.sigmaaldrich.com
M. F. C4H10O2S2 Telephone 1-800-521-8956
M. W. 154.251 Fax
Purity ≥99.5% (RT) Email
Storage Chembase ID: 1466

SYNONYMS

Title
DL-二硫苏糖醇
IUPAC name
(2S,3S)-1,4-disulfanylbutane-2,3-diol
IUPAC Traditional name
dithiothreitol
Synonyms
threo-1,4-Dimercapto-2,3-butanediol
Cleland’s reagent
DTT
1,4-二巯基苏糖醇
克莱兰氏试剂

DATABASE IDS

MDL Number MFCD00004877
PubChem SID 24867426
EC Number 222-468-7
CAS Number 3483-12-3
Beilstein Number 1719757

PROPERTIES

Antion Traces sulfate (SO42-): ≤50 mg/kg
Cation Traces Al: ≤5 mg/kg
Cation Traces As: ≤0.5 mg/kg
Cation Traces Ba: ≤5 mg/kg
Cation Traces Bi: ≤5 mg/kg
Cation Traces Ca: ≤10 mg/kg
Cation Traces Cd: ≤5 mg/kg
Cation Traces Co: ≤5 mg/kg
Cation Traces Cr: ≤5 mg/kg
Cation Traces Cu: ≤5 mg/kg
Cation Traces Fe: ≤5 mg/kg
Cation Traces K: ≤50 mg/kg
Cation Traces Li: ≤5 mg/kg
Cation Traces Mg: ≤5 mg/kg
Cation Traces Mn: ≤5 mg/kg
Cation Traces Mo: ≤5 mg/kg
Cation Traces Na: ≤100 mg/kg
Cation Traces Ni: ≤5 mg/kg
Cation Traces Pb: ≤5 mg/kg
Cation Traces Sr: ≤5 mg/kg
Cation Traces Zn: ≤5 mg/kg
Grade for molecular biology
Impurities DNases, none detected
Impurities RNases, none detected
Impurities insoluble matter, passes filter test
Impurities phosphatases, none detected
Impurities proteases, none detected
Impurities ≤0.3% 4,5-dihydroxy-1,2-dithiane
λ 0.1 M in H2O
Linear Formula HSCH2CH(OH)CH(OH)CH2SH
Product Line BioUltra
Purity ≥99.5% (RT)
Absorption Wavelength λ: 260 nm Amax: 0.400
Absorption Wavelength λ: 280 nm Amax: 0.100
Flash Point 113 °C
Flash Point 235.4 °F
Melting Point 41-44 °C(lit.)
pH 4.0-6.5 (25 °C, 0.1 M in H2O)
Solubility H2O: soluble0.1 M at 20 °C, clear, colorless
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H302-H315-H319-H335
European Hazard Symbols Harmful Harmful (Xn)
MSDS Link Download
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Precautionary statements P261-P305 + P351 + P338
Risk Statements 22-36/37/38
RTECS EK1610000
Safety Statements 26-36
Storage Temperature 2-8°C
German water hazard class 3

DETAILS

Description (English)
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1
Description (简体中文)
Application
用于将 -SH 基保持在还原态的绝佳试剂;定量还原二硫化物。在 SDS-PAGE 前,DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常,使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1

REFERENCES