S-Gal®

• Catalog No.: S9811
• CAS Number: 182805-65-8
• M. F.: C19H22O9
• M. W.: 394.37258

SYNONYMS

• IUPAC name: 2-hydroxy-3-{[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-6H,7H,8H,9H,10H-cyclohexa[c]chromen-6-one
• IUPAC Traditional name: 2-hydroxy-3-{[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-7H,8H,9H,10H-cyclohexa[c]chromen-6-one
• Synonyms: 3,4-Cyclohexenoesculetin β-D-galactopyranoside

DATABASE IDS

• CAS Number: 182805-65-8

PROPERTIES

• Empirical Formula (Hill Notation): C19H22O9
• Apperance: powder
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• German water hazard class: 3

DETAILS

Description (English)

Application
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Caution
For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.
Features and Benefits
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.
Other Notes
Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
Reconstitution
Add to agar media pre-autoclaving at a recommended concentration of 300 mg S-Gal/L of media, along with 500mg/L Ferric Ammonium Citrate (F5879). Stock solutions of S-Gal can be made by dissolving at 50mg/ml in dimethyl formamide and storing at -20 °C.
Legal Information
S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Description (简体中文)

Application
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Caution
For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.
Features and Benefits
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.
Other Notes
Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
Reconstitution
Add to agar media pre-autoclaving at a recommended concentration of 300 mg S-Gal/L of media, along with 500mg/L Ferric Ammonium Citrate (F5879). Stock solutions of S-Gal can be made by dissolving at 50mg/ml in dimethyl formamide and storing at -20 °C.
法律信息
S-GAL is a registered trademark of Sigma-Aldrich Co. LLC