Tris Acetate-EDTA buffer

• Catalog No.: T6025
• M. F.: C16H31N3O13
• M. W.: 473.42964

SYNONYMS

• Title: Tris 乙酸盐 EDTA 缓冲液
• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; acetic acid
• IUPAC Traditional name: acetic acid; edta; tris buffer
• Synonyms: TAE 缓冲液; TAE buffer

DATABASE IDS

• EC Number: 231-791-2
• MDL Number: MFCD00236357

PROPERTIES

• Impurities: DNase, RNase, Protease, none detected
• Product Line: BioReagent
• Sterility: 0.2 μm filtered
• Suitability: suitable for electrophoresis
• Suitability: suitable for gel electrophoresis (after dilution to working concentration)
• Apperance: working solution
• Personal Protective Equipment: Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
• German water hazard class: 2

DETAILS

Description (English)

Application
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis.1 Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity.2 Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Description (简体中文)

Application
TAE 电泳缓冲液是 DNA 琼脂糖凝胶电泳最常用的缓冲液，还用于非变性 RNA 琼脂糖凝胶电泳。1双链 DNA 在 TAE 中往往比在其他缓冲液中移动得更快，但在长时电泳期间也会因缓冲液离子耗尽而无法泳动。在长时电泳期间进行缓冲液循环或缓冲液更换可弥补较低的缓冲能力。2稀释浓缩的 TAE 缓冲液得到 1× TAE 缓冲液，含有 40mM Tris 乙酸盐和 1mM EDTA，pH 为 8.3。1× TAE 缓冲液既可用于琼脂糖凝胶中，也可用作电泳缓冲液。为达到最大分辨率，建议外加电压低于 5V/cm（单位电极间的距离）。
Other Notes
40mM Tris 乙酸盐，pH 约为 8.3，含有 1mM EDTA。
Preparation Note
用 18 兆欧水制备的溶液
采用经生物技术性能认证的 Trizma 碱 (T6066) 和分子生物学试剂 EDTA (E5134) 制备。溶液还含有乙酸 (A6283)；粉末状混合物含有 Trizma 醋酸盐 (T1258)。