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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt_Molecular_structure_CAS_6578-06-9)
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Catalog No. B8503 Name Sigma Aldrich
CAS Number 6578-06-9 Website http://www.sigmaaldrich.com
M. F. C15H15BrClN2O4P Telephone 1-800-521-8956
M. W. 433.621361 Fax
Purity ≥99% (HPLC) Email
Storage Chembase ID: 102152

SYNONYMS

Title
5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐
IUPAC name
4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)oxy]phosphonic acid
IUPAC Traditional name
(5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine
Synonyms
BCIP® p-toluidine salt
BCIP® 对甲苯胺盐
X-phosphate p-toluidine salt

DATABASE IDS

PubChem SID 24892074
EC Number 229-506-1
MDL Number MFCD00040636
CAS Number 6578-06-9

PROPERTIES

Empirical Formula (Hill Notation) C8H6BrClNO4P · C7H9N
Purity ≥99% (HPLC)
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H315-H319-H335
European Hazard Symbols Irritant Irritant (Xi)
MSDS Link Download
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
GHS Precautionary statements P261-P305 + P351 + P338
Risk Statements 36/37/38
Safety Statements 26
Storage Temperature -20°C
German water hazard class 3

DETAILS

Description (English)
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Description (简体中文)
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

REFERENCES