o-Dianisidine dihydrochloride

• Catalog No.: D9154
• CAS Number: 20325-40-0
• M. F.: C14H18Cl2N2O2
• M. W.: 317.21092

SYNONYMS

• Title: 邻联茴香胺 二盐酸盐
• IUPAC name: 4-(4-amino-3-methoxyphenyl)-2-methoxyaniline dihydrochloride
• IUPAC Traditional name: dianisidine dihydrochloride
• Synonyms: 3,3'-二甲氧基联苯胺 二盐酸盐; 固蓝 B; 3,3′-Dimethoxybenzidine dihydrochloride; Fast Blue B

DATABASE IDS

• CAS Number: 20325-40-0
• MDL Number: MFCD00012488
• PubChem SID: 24894263
• EC Number: 243-737-5
• Beilstein Number: 3917996

PROPERTIES

• Empirical Formula (Hill Notation): C14H16N2O2 · 2HCl
• Apperance: tablet
• GHS Signal Word: Danger
• GHS Hazard statements: H302-H350
• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
• GHS Precautionary statements: P201-P308 + P313
• Risk Statements: 45-22
• RTECS: DD1050000
• Safety Statements: 53-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Reconstitution
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.
Specificity
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Warning
Probably carcinogenic.

Description (简体中文)

Reconstitution
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.
Specificity
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Warning
Probably carcinogenic.