Biotin-maleimide

• Catalog No.: B1267
• M. F.: C20H29N5O5S
• M. W.: 451.53976
• Purity: ≥95% (TLC)

SYNONYMS

• IUPAC name: N'-{5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoyl}-6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanehydrazide
• IUPAC Traditional name: N'-{5-[(3aS,4S,6aR)-2-oxo-hexahydrothieno[3,4-d]imidazolidin-4-yl]pentanoyl}-6-(2,5-dioxopyrrol-1-yl)hexanehydrazide
• Synonyms: N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide

PROPERTIES

• Empirical Formula (Hill Notation): C20H29N5O5S
• Purity: ≥95% (TLC)
• Apperance: powder
• Solubility: acetic acid: soluble20 mg/mL
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides1.
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.
General description
Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups2.

Description (简体中文)

Application
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.