Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate

• Catalog No.: G0635
• CAS Number: 148892-91-5
• M. F.: C10H14N6Na3O13P3
• M. W.: 588.141153
• Purity: ≥85% (HPLC)

SYNONYMS

• IUPAC name: trisodium hydrogen {[({[(2R,3S,4R,5R)-5-(2-amino-6-hydroxy-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxy)phosphinato]amino}phosphonate
• IUPAC Traditional name: trisodium hydrogen ({[(2R,3S,4R,5R)-5-(2-amino-6-hydroxypurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxyphosphinato)aminophosphonate
• Synonyms: Gpp(NH)p; 5′-Guanylyl-imidodiphosphate trisodium salt hydrate; β:γ-Imidoguanosine 5′-triphosphate trisodium salt hydrate; GMP-PNP

DATABASE IDS

• MDL Number: MFCD00084770
• CAS Number: 148892-91-5
• PubChem SID: 24895020

PROPERTIES

• Empirical Formula (Hill Notation): C10H14N6Na3O13P3 · xH2O
• Purity: ≥85% (HPLC)
• Shipped in: dry ice
• Apperance: white powder
• Solubility: H2O: soluble50 mg/mL
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26-36/37
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.
Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.
Biochem/physiol Actions
Binds and irreversibly activates G proteins.1 Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.2,3

Description (简体中文)

Application
Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.
Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.
Biochem/physiol Actions
Binds and irreversibly activates G proteins.1 Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.2,3