N,N′-Bis(acryloyl)cystamine

• Catalog No.: A4929
• CAS Number: 60984-57-8
• M. F.: C10H16N2O2S2
• M. W.: 260.37624

SYNONYMS

• IUPAC name: N-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide
• IUPAC Traditional name: N-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide
• Synonyms: BAC; 2,2′-(Bisacrylamino)diethyl disulfide; Bis(2-acrylamidoethyl) disulfide

DATABASE IDS

• EC Number: 262-546-8
• PubChem SID: 24890926
• CAS Number: 60984-57-8
• MDL Number: MFCD00036225

PROPERTIES

• Empirical Formula (Hill Notation): C10H16N2O2S2
• Product Line: BioReagent
• Suitability: suitable for electrophoresis
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Description (English)

Application
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.