Chloramphenicol

• Catalog No.: C7795
• CAS Number: 56-75-7
• M. F.: C11H12Cl2N2O5
• M. W.: 323.12938

SYNONYMS

• Title: 氯霉素
• IUPAC name: 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide
• IUPAC Traditional name: chloromycetin
• Synonyms: D(-)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide; D-(-)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol; D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide; D-(-)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide; Chloromycetin

DATABASE IDS

• MDL Number: MFCD00078159
• Beilstein Number: 2225532
• PubChem SID: 24892989
• CAS Number: 56-75-7
• EC Number: 200-287-4

PROPERTIES

• Linear Formula: Cl2CHCONHCH(CH2OH)CH(OH)C6H4NO2
• Sterility: γ-irradiated
• Gene Information: human ... CYP1A2(1544)
• Melting Point: 148-150 °C(lit.)
• Solubility: absolute ethanol: soluble5-20 mg/mL (as a stock solution)
• Solubility: methanol: soluble (as a stock solution)
• GHS Signal Word: Danger
• GHS Hazard statements: H350
• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
• GHS Precautionary statements: P201-P308 + P313
• Risk Statements: 45
• RTECS: AB6825000
• Safety Statements: 53-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Use as a seletion agent for transformed cells containing chloramphenicol resistance genes. Recommended for use in molecular biology applications at 10-20 μg/ml.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Reconstitution
Prepare stock solutions directly in the vial at any concentration in the recommended range. Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 5 days.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Description (简体中文)

Application
Use as a seletion agent for transformed cells containing chloramphenicol resistance genes. Recommended for use in molecular biology applications at 10-20 μg/ml.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Reconstitution
直接用小瓶配制推荐范围内任意浓度的储存液。储存液应在 2-8°C 下保存。在 37°C 下可稳定 5 天。
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information