DAF-FM DA

• Catalog No.: D1946
• CAS Number: 254109-22-3
• M. F.: C25H18F2N2O7
• M. W.: 496.4164264
• Purity: ≥98% (HPLC)

SYNONYMS

• IUPAC name: 6'-(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3'-yl acetate
• IUPAC Traditional name: 6'-(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl acetate
• Synonyms: Diaminofluorescein-FM diacetate; DAF-FM DA; 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy); 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one

DATABASE IDS

• CAS Number: 254109-22-3
• MDL Number: MFCD16872050

PROPERTIES

• Empirical Formula (Hill Notation): C25H18F2N2O7
• Purity: ≥98% (HPLC)
• Shipped in: wet ice
• Apperance: white to off-white powder
• Fluorescence: λex 500 nm; λem 515 nm in DMSO
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Caution
Hygroscopic
Biochem/physiol Actions
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Description (简体中文)

Caution
Hygroscopic
Biochem/physiol Actions
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.