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Fluorescent Red Mega 500_Molecular_structure_CAS_540528-13-0)
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Fluorescent Red Mega 500

Catalog No. 56900 Name Sigma Aldrich
CAS Number 540528-13-0 Website http://www.sigmaaldrich.com
M. F. C31H36N2O8S Telephone 1-800-521-8956
M. W. 596.69114 Fax
Purity ≥90% (HPLC) Email
Storage Chembase ID: 154224

SYNONYMS

IUPAC name
1-(5-carboxypentyl)-2-{2-[7-(diethylamino)-4-hydroxy-2-oxo-2H-chromen-3-yl]ethenyl}-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
IUPAC Traditional name
1-(5-carboxypentyl)-2-{2-[7-(diethylamino)-4-hydroxy-2-oxochromen-3-yl]ethenyl}-3,3-dimethylindol-1-ium-5-sulfonate

DATABASE IDS

MDL Number MFCD06798196
CAS Number 540528-13-0
PubChem SID 24880461

PROPERTIES

Empirical Formula (Hill Notation) C31H36N2O8S
Product Line BioReagent
Purity ≥90% (HPLC)
Suitability suitable for fluorescence
Fluorescence λex 508 nm; λem 608 nm in 0.1 M phosphate pH 7.0
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Storage Temperature 2-8°C
German water hazard class 3

DETAILS

Description (English)
General description
Fluorescent red Mega 500 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This also means, that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.
Description (简体中文)
General description
Fluorescent red Mega 500 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This also means, that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.

REFERENCES