Puromycin dihydrochloride

• Catalog No.: P9620
• CAS Number: 58-58-2
• M. F.: C22H31Cl2N7O5
• M. W.: 544.43144
• Purity: >98% (HPLC)

SYNONYMS

• Title: Puromycin
• IUPAC name: (2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)-9H-purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide dihydrochloride
• IUPAC Traditional name: puromycin dihydrochloride

DATABASE IDS

• MDL Number: MFCD00012691
• CAS Number: 58-58-2

PROPERTIES

• Biological Source: from Streptomyces alboniger
• Concentration: 10 mg/mL in H2O
• Purity: >98% (HPLC)
• Quality Level: PREMIUM
• Shipped in: wet ice
• Sterility: 0.2 μm filtered
• Suitability: cell culture tested
• Personal Protective Equipment: Eyeshields, Faceshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
• Storage Temperature: -20°C
• German water hazard class: 2

DETAILS

Description (English)

Application
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2.
Biochem/physiol Actions
Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Suitability
Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Description (简体中文)

Application
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2.
Biochem/physiol Actions
Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Suitability
Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information