Home > Compound List > Product Information
Fluorescent Red Mega 520_Molecular_structure_CAS_540528-09-4)
Click picture or here to close

Fluorescent Red Mega 520

Catalog No. 41306 Name Sigma Aldrich
CAS Number 540528-09-4 Website http://www.sigmaaldrich.com
M. F. C26H30N2O7S Telephone 1-800-521-8956
M. W. 514.5906 Fax
Purity ≥97.0% (HPLC) Email
Storage Chembase ID: 154150

SYNONYMS

IUPAC name
1-(5-carboxypentyl)-4-{2-[7-(diethylamino)-2-oxo-2H-chromen-3-yl]ethenyl}pyridin-1-ium-3-sulfonate
IUPAC Traditional name
1-(5-carboxypentyl)-4-{2-[7-(diethylamino)-2-oxochromen-3-yl]ethenyl}pyridin-1-ium-3-sulfonate
Synonyms
6-[4-(7-Diethylamino-2-oxo-1-benzopyran-3-ylvinyl)-3-sulfonatopyridinio]hexanoic acid

DATABASE IDS

CAS Number 540528-09-4
MDL Number MFCD06798178
PubChem SID 24865840

PROPERTIES

Empirical Formula (Hill Notation) C26H30N2O7S
Product Line BioReagent
Purity ≥97.0% (HPLC)
Suitability suitable for fluorescence
Fluorescence λex 527 nm; λem 663 nm in 0.1 M phosphate pH 7.0
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Storage Temperature 2-8°C
German water hazard class 3

DETAILS

Description (English)
General description
Fluorescent red Mega 520 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stokes shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This also means that it can be excited with the same light source or filter arrangement as fluorescein and other widely used dyes, as well as other Mega dyes. Due to the different emission wavelengths several different labels can be detected independently in the same experiment. Due to the small size of the Mega labels and the direct conversion of excitation light into emission without using a FRET mechanism, there is a variety of possible applications including DNA sequencing and FISH microscopy.
Description (简体中文)
General description
Fluorescent red Mega 520 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stokes shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This also means that it can be excited with the same light source or filter arrangement as fluorescein and other widely used dyes, as well as other Mega dyes. Due to the different emission wavelengths several different labels can be detected independently in the same experiment. Due to the small size of the Mega labels and the direct conversion of excitation light into emission without using a FRET mechanism, there is a variety of possible applications including DNA sequencing and FISH microscopy.

REFERENCES