G 418 disulfate salt solution

• Catalog No.: G8168
• CAS Number: 108321-42-2
• M. F.: C20H44N4O18S2
• M. W.: 692.70936

SYNONYMS

• IUPAC name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• IUPAC Traditional name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• Synonyms: Antibiotic G418

DATABASE IDS

• CAS Number: 108321-42-2
• MDL Number: MFCD00058314
• PubChem SID: 24895333

PROPERTIES

• Concentration: 50 mg/mL in H2O
• Empirical Formula (Hill Notation): C20H40N4O10 · 2H2SO4
• Impurities: endotoxin, tested
• Product Line: BioReagent
• Sterility: sterile-filtered
• Suitability: suitable for cell culture
• GHS Signal Word: Danger
• GHS Hazard statements: H317-H334
• European Hazard Symbols: Harmful (Xn)
• GHS Precautionary statements: P261-P280-P342 + P311
• Risk Statements: 42/43
• Safety Statements: 23-36/37-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Physical form
50 mg/mL in tissue culture grade water
Application
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Description (简体中文)

Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Physical form
50 mg/mL in tissue culture grade water
Application
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information