Application
Used for selection of ampr transformed cells. Use to study the role of penicillin-sensitive transpeptidases in cell wall biosynthesis.
Biochem/physiol Actions
The antibiotic carbenicillin, an ampicillin analog, is a commonly used selection agent that binds and inhibits enzymes involved in the synthesis of the bacterial cell wall. It is active against most isolates of Pseudomonas aerogenosa and certain indole-positive Proteus strains that are resistant to ampicillin. The gene conferring resistance to ampicillin and its analogs, ampr, codes for the enzyme β-lactamase. Carbenicillin is less sensitive to β-lactamase than ampicillin. In addition it has a superior stability at low pH. Experiments have shown that the use of carbenicillin in place of ampicillin helps prevent overgrowth of satellite colonies. Effective concentration: 50 to 100 μg/ml.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information |