2-Mercaptoethanol

• Catalog No.: 63689
• CAS Number: 60-24-2
• M. F.: C2H6OS
• M. W.: 78.13344
• Purity: ≥99.0% (GC)

SYNONYMS

• Title: 2-巯基乙醇
• IUPAC name: 2-sulfanylethan-1-ol
• IUPAC Traditional name: β-mercaptoethanol
• Synonyms: 2-羟基乙硫醇; BME; β-Mercaptoethanol; Thioethylene glycol; β-巯基乙醇; 硫代乙二醇; 2-Hydroxyethylmercaptan

DATABASE IDS

• Beilstein Number: 773648
• CAS Number: 60-24-2
• EC Number: 200-464-6
• MDL Number: MFCD00004890
• PubChem SID: 24882813

PROPERTIES

• Cation Traces: Al: ≤0.5 mg/kg
• Cation Traces: Ba: ≤0.1 mg/kg
• Cation Traces: Bi: ≤0.1 mg/kg
• Cation Traces: Ca: ≤0.5 mg/kg
• Cation Traces: Cd: ≤0.05 mg/kg
• Cation Traces: Co: ≤0.02 mg/kg
• Cation Traces: Cr: ≤0.02 mg/kg
• Cation Traces: Cu: ≤0.02 mg/kg
• Cation Traces: Fe: ≤0.1 mg/kg
• Cation Traces: K: ≤0.5 mg/kg
• Cation Traces: Li: ≤0.1 mg/kg
• Cation Traces: Mg: ≤0.1 mg/kg
• Cation Traces: Mn: ≤0.02 mg/kg
• Cation Traces: Mo: ≤0.1 mg/kg
• Cation Traces: Na: ≤1 mg/kg
• Cation Traces: Ni: ≤0.05 mg/kg
• Cation Traces: Pb: ≤0.1 mg/kg
• Cation Traces: Sr: ≤0.1 mg/kg
• Cation Traces: Zn: ≤0.1 mg/kg
• Concentration: 14.3 M (pure liquid)
• Grade: for molecular biology
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• λ: 0.5 M in H2O
• Linear Formula: HSCH2CH2OH
• Product Line: BioUltra
• Purity: ≥99.0% (GC)
• Target Organ: Heart, Liver
• Absorption Wavelength: λ: 260 nm Amax: 1.5
• Absorption Wavelength: λ: 280 nm Amax: 0.3
• Boiling Point: 157 °C(lit.)
• Density: 1.114 g/mL at 25 °C(lit.)
• Flash Point: 68 °C
• Flash Point: 154.4 °F
• Refractive Index: n20/D 1.500(lit.)
• Refractive Index: n20/D 1.501
• Solubility: H2O: soluble0.5 M at 20 °C, clear, colorless
• Vapor Density: 2.69 (vs air)
• Vapor Pressure: 1 mmHg ( 20 °C)
• Explode Limits: 18 %
• GHS Signal Word: Danger
• GHS Hazard statements: H301 + H331-H310-H315-H317-H318-H373-H410
• European Hazard Symbols: Toxic (T)
• European Hazard Symbols: Nature polluting (N)
• Personal Protective Equipment: Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P261-P273-P280-P301 + P310-P302 + P350-P305 + P351 + P338
• RID/ADR: UN 2966 6.1/PG 2
• Risk Statements: 23/24/25-38-41-43-48/22-50/53
• RTECS: KL5600000
• Safety Statements: 26-36/37/39-45-60-61
• Storage Temperature: 2-8°C
• Hazard Class: 6.1
• UN Number: 2966
• Packing Group: 2
• German water hazard class: 3

DETAILS

Description (English)

Application
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds1

Description (简体中文)

Application
BME 用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键，通常以 5% 的浓度包含在用于 SDS-PAGE 的样本缓冲液中。裂解分子间（亚基之间）的二硫键，从而使蛋白质亚基在 SDS-PAGE 上各自分开。裂解分子内（亚基内）的二硫键从而使亚基完全变性，以便每个肽按照其链长迁移而不受二级结构的影响。
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds1