G 418 disulfate salt

• Catalog No.: A8601
• CAS Number: 108321-42-2
• M. F.: C20H44N4O18S2
• M. W.: 692.70936

SYNONYMS

• Title: G 418 二硫酸盐
• IUPAC name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• IUPAC Traditional name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• Synonyms: 抗生物質G418; Antibiotic G418

DATABASE IDS

• CAS Number: 108321-42-2
• MDL Number: MFCD00058314

PROPERTIES

• Empirical Formula (Hill Notation): C20H40N4O10 · 2H2SO4
• Grade: Biotechnology Performance Certified
• Impurities: endotoxin, tested
• Suitability: suitable for cell culture
• Suitability: suitable for plant cell culture
• GHS Signal Word: Danger
• GHS Hazard statements: H317-H334
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• GHS Precautionary statements: P261-P280-P342 + P311
• Risk Statements: 42/43
• Safety Statements: 22-36/37-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Used for selection of G418 resistant transformed cells in molecular biology applications.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Application
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths1. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene2.
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml.Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those that are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.

Description (English)

Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Used for selection of G418 resistant transformed cells in molecular biology applications.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Application
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths1. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene2.
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml.Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those that are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.