N-(4-Anilino-1-naphthyl)maleimide

• Catalog No.: 10420
• CAS Number: 50539-45-2
• M. F.: C20H14N2O2
• M. W.: 314.33736
• Purity: ≥99.0% (HPLC)

SYNONYMS

• IUPAC name: 1-[4-(phenylamino)naphthalen-1-yl]-2,5-dihydro-1H-pyrrole-2,5-dione
• IUPAC Traditional name: 1-[4-(phenylamino)naphthalen-1-yl]pyrrole-2,5-dione
• Synonyms: 1-[4-(phenylamino)naphthyl]-1H-pyrrole-2,5-dione; 1-(4-Anilino-1-naphtyl)-3-pyrroline-2,5-dione; N-(1-Anilinonaphthyl-4)-maleimide; ANM; 1-[4-(phenylamino)naphthalen-1-yl]-2,5-dihydro-1H-pyrrole-2,5-dione; N-(1-Anilinophenyl)maleimide; N-[4-(Phenylamino)-1-naphtyl]maleimide

DATABASE IDS

• EC Number: 256-618-8
• MDL Number: MFCD00042694
• PubChem SID: 24846431
• CAS Number: 50539-45-2
• Beilstein Number: 1487865

PROPERTIES

• Empirical Formula (Hill Notation): C20H14N2O2
• Product Line: BioReagent
• Purity: ≥99.0% (HPLC)
• Suitability: suitable for fluorescence
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• German water hazard class: 3

DETAILS

Description (English)

Other Notes
Fluorescent hydrophobic probe for thiol groups in proteins; Review7,8
Application
ANM has been used to label muscle fibers to determine the conformational changes between the rigor state and the relaxed state 1>. This probe provides an excellent signal for monitoring changes in environment during protein refolding 2 because it′s fluorescence quantum yield is highly sensitive to solvent polarity 3. A non fluorescent compound which becomes strongly fluorescent upon selective reaction wih thiols 3. ANM can be used to monitor heterodimerization of the α and β subunits of luciferase. 2. Applied to monitor protein interactions using FRET 4 and protein conformational changes using nanosecond time-resolved fluorescence experiments 5. This probe has been used to determine the reactivity of the sulphydrl groups in tryptophanse 6.

Description (简体中文)

Other Notes
Fluorescent hydrophobic probe for thiol groups in proteins; Review7,8
Application
ANM has been used to label muscle fibers to determine the conformational changes between the rigor state and the relaxed state 1>. This probe provides an excellent signal for monitoring changes in environment during protein refolding 2 because it′s fluorescence quantum yield is highly sensitive to solvent polarity 3. A non fluorescent compound which becomes strongly fluorescent upon selective reaction wih thiols 3. ANM can be used to monitor heterodimerization of the α and β subunits of luciferase. 2. Applied to monitor protein interactions using FRET 4 and protein conformational changes using nanosecond time-resolved fluorescence experiments 5. This probe has been used to determine the reactivity of the sulphydrl groups in tryptophanse 6.