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3,3′,5,5′-Tetramethylbenzidine_Molecular_structure_CAS_54827-17-7)
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3,3′,5,5′-Tetramethylbenzidine

Catalog No. 860336 Name Sigma Aldrich
CAS Number 54827-17-7 Website http://www.sigmaaldrich.com
M. F. C16H20N2 Telephone 1-800-521-8956
M. W. 240.3434 Fax
Purity ≥99% Email
Storage Chembase ID: 74451

SYNONYMS

Title
3,3′,5,5′-四甲基联苯胺
IUPAC name
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
IUPAC Traditional name
tetramethylbenzidine
Synonyms
TMB
TMB substrate
Sure Blue TMB
TMB Blotting Plus
BM blue

DATABASE IDS

CAS Number 54827-17-7
Beilstein Number 2808541
EC Number 259-364-6
MDL Number MFCD00007748
PubChem SID 24888505

PROPERTIES

Linear Formula [-C6H2(CH3)2-4-NH2]2
Purity ≥99%
Melting Point 168-171 °C(lit.)
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H315-H319-H335
European Hazard Symbols Irritant Irritant (Xi)
MSDS Link Download
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Precautionary statements P261-P305 + P351 + P338
Risk Statements 36/37/38
RTECS DV2300000
Safety Statements 26-36
Storage Temperature 2-8°C
German water hazard class 3

DETAILS

Description (English)
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8
Packaging
1, 5, 25 g in glass bottle
100 mg in glass bottle
Description (简体中文)
Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性),适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物,该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色),并在 450nm 处读取数据。一种灵敏的特异性试剂,用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8
包装
1, 5, 25 g in glass bottle
100 mg in glass bottle

REFERENCES