Application 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8 Packaging 1, 5, 25 g in glass bottle 100 mg in glass bottle |
Application 本品为联苯胺1的非致癌性替代品(Ames 试验阴性),适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物,该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色),并在 450nm 处读取数据。一种灵敏的特异性试剂,用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8 包装 1, 5, 25 g in glass bottle 100 mg in glass bottle |