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Fast Green FCF_Molecular_structure_CAS_2353-45-9)
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Fast Green FCF

Catalog No. 861154 Name Sigma Aldrich
CAS Number 2353-45-9 Website http://www.sigmaaldrich.com
M. F. C37H34N2Na2O10S3 Telephone 1-800-521-8956
M. W. 808.8478 Fax
Purity Email
Storage Chembase ID: 109627

SYNONYMS

IUPAC name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzene-1-sulfonate
IUPAC Traditional name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzenesulfonate
Synonyms
Food green 3

DATABASE IDS

Beilstein Number 5718212
CAS Number 2353-45-9
Color Index Number 42053
EC Number 219-091-5
MDL Number MFCD00013053

PROPERTIES

Compostion Dye content, 90%
Linear Formula C37H34N2O10S3Na2
Absorption Wavelength λmax 427 nm
Absorption Wavelength λmax 622 nm (2nd)
Melting Point 290 °C (dec.)(lit.)
GHS Pictograms GHS08
GHS Signal Word Warning
GHS Hazard statements H341
European Hazard Symbols Harmful Harmful (Xn)
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves
GHS Precautionary statements P281
Risk Statements 68
RTECS BQ4425000
Safety Statements 36/37
German water hazard class 2

DETAILS

Description (English)
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Description (简体中文)
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

REFERENCES