5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

• Catalog No.: B9146
• CAS Number: 7240-90-6
• M. F.: C14H15BrClNO6
• M. W.: 408.629
• Purity: ≥98%

SYNONYMS

• IUPAC name: (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• IUPAC Traditional name: X-gal
• Synonyms: X-Gal; BCIG; 5-Bromo-4-chloro-3-indolyl β-D-galactoside

DATABASE IDS

• EC Number: 230-640-8
• Beilstein Number: 1402009
• PubChem SID: 24892124
• CAS Number: 7240-90-6
• MDL Number: MFCD00005666

PROPERTIES

• Purity: ≥98%
• Suitability: suitable for identification of lac+ bacterial colonies
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Reconstitution
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at -20 °C and protected from light. Sterilization is not required.