o-Phenylenediamine

• Catalog No.: P5412
• CAS Number: 95-54-5
• M. F.: C6H8N2
• M. W.: 108.14112

SYNONYMS

• Title: 邻苯二胺
• IUPAC name: benzene-1,2-diamine
• IUPAC Traditional name: o-phenylenediamine
• Synonyms: 1,2-二氨基苯; 1,2-苯二胺; OPD

DATABASE IDS

• Beilstein Number: 606074
• PubChem SID: 24898638
• CAS Number: 95-54-5
• EC Number: 202-430-6
• MDL Number: MFCD00007721

PROPERTIES

• Apperance: tablet
• Boiling Point: 256-258 °C
• Flash Point: 136 °C
• Flash Point: 276.8 °F
• Fluorescence: λex 337 nm; λem 417 nm (after derivatization)
• Melting Point: 100-102 °C
• Vapor Density: 3.7 (vs air)
• Vapor Pressure: 0.01 mmHg ( 25 °C)
• GHS Signal Word: Danger
• GHS Hazard statements: H301-H312-H317-H319-H332-H341-H351-H410
• European Hazard Symbols: Toxic (T)
• European Hazard Symbols: Nature polluting (N)
• GHS Precautionary statements: P273-P280-P301 + P310-P305 + P351 + P338-P501
• RID/ADR: UN 1673 6.1/PG 3
• Risk Statements: 20/21-25-36-40-43-50/53-68
• Safety Statements: 28-36/37-45-60-61
• Storage Temperature: 2-8°C
• Hazard Class: 6.1
• UN Number: 1673
• Packing Group: 3
• German water hazard class: 3

DETAILS

Description (English)

Application
o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.