2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

• Catalog No.: A9941
• CAS Number: 30931-67-0
• M. F.: C18H24N6O6S4
• M. W.: 548.67976

SYNONYMS

• Title: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 二铵盐
• IUPAC name: diammonium (2Z)-3-ethyl-2-{2-[(2Z)-3-ethyl-6-sulfonato-2,3-dihydro-1,3-benzothiazol-2-ylidene]hydrazin-1-ylidene}-2,3-dihydro-1,3-benzothiazole-6-sulfonate
• IUPAC Traditional name: diammonium (2Z)-3-ethyl-2-{2-[(2Z)-3-ethyl-6-sulfonato-1,3-benzothiazol-2-ylidene]hydrazin-1-ylidene}-1,3-benzothiazole-6-sulfonate
• Synonyms: AzBTS-(NH4)2; Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

DATABASE IDS

• MDL Number: MFCD00010404
• Beilstein Number: 7329461
• PubChem SID: 24891410
• CAS Number: 30931-67-0
• EC Number: 250-396-6

PROPERTIES

• Apperance: tablet
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• RTECS: DL7002000
• Safety Statements: 26-36
• German water hazard class: 3

DETAILS

Description (English)

Application
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Reconstitution
Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide