4′,6-Diamidino-2-phenylindole dihydrochloride

• Catalog No.: D8417
• CAS Number: 28718-90-3
• M. F.: C16H17Cl2N5
• M. W.: 350.24568
• Purity: ≥98% (HPLC and TLC)

SYNONYMS

• IUPAC name: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• IUPAC Traditional name: DAPI dihydrochloride
• Synonyms: DAPI dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride

DATABASE IDS

• EC Number: 249-186-7
• CAS Number: 28718-90-3
• MDL Number: MFCD00012681
• Beilstein Number: 4894417
• PubChem SID: 24894213

PROPERTIES

• Purity: ≥98% (HPLC and TLC)
• Suitability: suitable for cell culture
• Suitability: suitable for fluorescence
• Absorption Wavelength: ε/263 nm, H2O 30
• Apperance: powder
• Fluorescence: λex 340 nm; λem 488 nm (nur DAPI)
• Fluorescence: λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
• Solubility: H2O: soluble20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
• Solubility: PBS: insoluble
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caution
Protect from light.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.