Hygromycin B solution from Streptomyces hygroscopicus

• Catalog No.: H5527
• CAS Number: 31282-04-9
• M. F.: C20H37N3O13
• M. W.: 527.52008
• Purity: ≥60% (HPAE)

SYNONYMS

• Title: 潮霉素 B 溶液 来源于吸水链霉菌
• IUPAC name: (2S,3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)-tetrahydro-3aH-spiro[[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• IUPAC Traditional name: (2S,3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)-tetrahydro-3aH-spiro[[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

DATABASE IDS

• MDL Number: MFCD06795479
• CAS Number: 31282-04-9
• PubChem SID: 24895698
• Beilstein Number: 6755837

PROPERTIES

• Concentration: 45-60 mg/mL in H2O
• Purity: ≥60% (HPAE)
• Sterility: γ-irradiated
• GHS Signal Word: Danger
• GHS Hazard statements: H301 + H311-H318-H330-H334
• European Hazard Symbols: Highly toxic (T+)
• Personal Protective Equipment: Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P260-P280-P284-P301 + P310-P305 + P351 + P338-P310
• RID/ADR: UN 2810 6.1/PG 1
• Risk Statements: 26/27/28-41-42/43
• Safety Statements: 23-26-28-36/37/39-45
• Storage Temperature: 2-8°C
• Hazard Class: 6.1
• UN Number: 2810
• Packing Group: 1
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
在许多细胞培养应用中，潮霉素已用于在转移潮霉素磷酸转移酶基因后选择耐药稳定的转染子。
Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. It is used in gene transfer experiments as a selection agent. Product H5527 is a solution that is ≥60% (HPAE), 45-60 mg/mL in H2O, and γ-irradiated .
Biochem/physiol Actions
抗菌剂和抗真菌剂。作用方式：在原核生物和真核生物中，通过诱导 mRNA 模板的误读而抑制蛋白质合成。它可选择性透入因病毒感染而变得可渗透的细胞。
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information