2-Nitrophenyl β-D-galactopyranoside

• Catalog No.: N1127
• CAS Number: 369-07-3
• M. F.: C12H15NO8
• M. W.: 301.2494
• Purity: ≥98% (enzymatic)

SYNONYMS

• IUPAC name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
• IUPAC Traditional name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
• Synonyms: ONPG; o-Nitrophenyl β-D-galactopyranoside

DATABASE IDS

• EC Number: 206-716-1
• PubChem SID: 24897461
• Beilstein Number: 92207
• MDL Number: MFCD00063255
• CAS Number: 369-07-3

PROPERTIES

• Purity: ≥98% (enzymatic)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (简体中文)

包装
1 g in poly tube
250 mg in poly bottle
5, 25 g in poly bottle
500 mg in poly tube
Substrates
Chromogenic substrate for β-galactosidase
Application
2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.
Biochem/physiol Actions
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.