Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate

• Catalog No.: A2647
• CAS Number: 25612-73-1(freeacid)
• M. F.: C10H15Li4N6O13P3
• M. W.: 547.943783
• Purity: ≥93% (HPLC)

SYNONYMS

• IUPAC name: tetralithium(1+) ion hydrate {[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxy)phosphinato]amino}phosphonate
• IUPAC Traditional name: tetralithium(1+) ion hydrate ({[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxyphosphinato)aminophosphonate
• Synonyms: β,γ-Imidoadenosine 5′-triphosphate lithium salt hydrate; Adenylyl imidodiphosphate lithium salt hydrate; ATP[β,γ-NH]; AMP-PNP; App(NH)p

DATABASE IDS

• CAS Number: 25612-73-1(freeacid)
• Beilstein Number: 6047109
• PubChem SID: 24890685

PROPERTIES

• Purity: ≥93% (HPLC)
• Shipped in: dry ice
• Apperance: white powder
• Solubility: H2O: soluble50 mg/mL
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Biochem/physiol Actions
A non-hydrolyzable ATP analog. AMP-PNP competitively inhibits ATP-dependent enzyme systems, such as glutamine synthetase. It inhibits a number of enzymes which require the hydrolysis of ATP such as DNA topoisomerase II, SV40 large T-antigen helicase and a number of kinases. Blocks ATP-sensitive calcium-dependent potassium channels.
Caution
AMP-PNP is very unstable in acidic conditions; rapidly hydrolyzes to the phosphoramidate and inorganic phosphate.
Reconstitution
A 10 mg/mL stock solution may be made, aliquoted and stored at -70 °C for up to 3 months.
Application
Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has been used in a study to research the effects of monovalent cations Li+, Na+, K+, NH4+, Rb+ and Cs+ on the solid and solution structures of the nucleic acid components. Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has also been used in a study to determine that antidepressants inhibit interferon-γ-induced microglial production of IL-6 and nitric oxide.