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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt_Molecular_structure_CAS_6578-06-9)
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Catalog No. B0274 Name Sigma Aldrich
CAS Number 6578-06-9 Website http://www.sigmaaldrich.com
M. F. C15H15BrClN2O4P Telephone 1-800-521-8956
M. W. 433.621361 Fax
Purity Email
Storage Chembase ID: 102152

SYNONYMS

Title
5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐
IUPAC name
4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)oxy]phosphonic acid
IUPAC Traditional name
(5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine
Synonyms
BCIP® 对甲苯胺盐
X-phosphate p-toluidine salt

DATABASE IDS

EC Number 229-506-1
MDL Number MFCD00040636
PubChem SID 24891510
CAS Number 6578-06-9

PROPERTIES

Apperance tablet
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H315-H319-H335
European Hazard Symbols Irritant Irritant (Xi)
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
GHS Precautionary statements P261-P305 + P351 + P338
Risk Statements 36/37/38
Safety Statements 26
Storage Temperature -20°C
German water hazard class 3

DETAILS

Description (简体中文)
Quantity
Contains 25 mg substrate per tablet.
Specificity
Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

REFERENCES