G 418 disulfate salt

• Catalog No.: G5013
• CAS Number: 108321-42-2
• M. F.: C20H44N4O18S2
• M. W.: 692.70936

SYNONYMS

• Title: G 418 二硫酸盐
• IUPAC name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• IUPAC Traditional name: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• Synonyms: 抗生物質G418

DATABASE IDS

• CAS Number: 108321-42-2
• MDL Number: MFCD00058314
• PubChem SID: 24895193

PROPERTIES

• Potency: ≥450 μg per mg (dried basis)
• Apperance: white powder
• Solubility: H2O: soluble50 mg/mL
• GHS Signal Word: Danger
• GHS Hazard statements: H317-H334
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• GHS Precautionary statements: P261-P280-P342 + P311
• Risk Statements: 42/43
• Safety Statements: 22-36/37-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Caution
Stable at 37 °C for 8 days.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Reconstitution
Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months.
Application
Blocks protein synthesis by interfering with ribosomal function. Not normally used as a standard antibiotic, but rather for selection and maintenance of prokaryotic and eukaryotic cells stably transfected with neomycin resistance genes. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. The resistance genes are APH (3′) II or aminoglycoside phosphotransferase 3′(II) and aminoglycoside phosphotranferase 3′ (I) or APH (3′) I. The following concentrations of active ingredient may be used as a guide for selection: mammalian cells (400 ug/ml), plants (10 ug/ml), yeasts (500 ug/ml), and dictyostelium (10 ug/ml). Cells have variable resistance and multiplying cells will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. Varying concentrations should be tested. Additionally, fibroblast contamination in mixed cultures may be suppressed by using 100 ug/ml of the active ingredient for 2 days.
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information