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G 418 disulfate salt_Molecular_structure_CAS_108321-42-2)
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G 418 disulfate salt

Catalog No. G5013 Name Sigma Aldrich
CAS Number 108321-42-2 Website http://www.sigmaaldrich.com
M. F. C20H44N4O18S2 Telephone 1-800-521-8956
M. W. 692.70936 Fax
Purity Email
Storage Chembase ID: 131635


G 418 二硫酸盐
IUPAC name
(2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
IUPAC Traditional name
(2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)


CAS Number 108321-42-2
MDL Number MFCD00058314
PubChem SID 24895193


Potency ≥450 μg per mg (dried basis)
Apperance white powder
Solubility H2O: soluble50 mg/mL
GHS Pictograms GHS08
GHS Signal Word Danger
GHS Hazard statements H317-H334
European Hazard Symbols Harmful Harmful (Xn)
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
GHS Precautionary statements P261-P280-P342 + P311
Risk Statements 42/43
Safety Statements 22-36/37-45
Storage Temperature 2-8°C
German water hazard class 3


Description (English)
Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Stable at 37 °C for 8 days.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months.
Blocks protein synthesis by interfering with ribosomal function. Not normally used as a standard antibiotic, but rather for selection and maintenance of prokaryotic and eukaryotic cells stably transfected with neomycin resistance genes. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. The resistance genes are APH (3′) II or aminoglycoside phosphotransferase 3′(II) and aminoglycoside phosphotranferase 3′ (I) or APH (3′) I. The following concentrations of active ingredient may be used as a guide for selection: mammalian cells (400 ug/ml), plants (10 ug/ml), yeasts (500 ug/ml), and dictyostelium (10 ug/ml). Cells have variable resistance and multiplying cells will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. Varying concentrations should be tested. Additionally, fibroblast contamination in mixed cultures may be suppressed by using 100 ug/ml of the active ingredient for 2 days.
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information