Hygromycin B from Streptomyces hygroscopicus

• Catalog No.: H3274
• CAS Number: 31282-04-9
• M. F.: C20H37N3O13
• M. W.: 527.52008
• Purity: ≥60% (HPAE)

SYNONYMS

• Title: 潮霉素 B 来源于吸水链霉菌
• IUPAC name: (2S,3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)-tetrahydro-3aH-spiro[[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• IUPAC Traditional name: (2S,3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)-tetrahydro-3aH-spiro[[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

DATABASE IDS

• PubChem SID: 24895568
• MDL Number: MFCD06795479
• CAS Number: 31282-04-9
• EC Number: 250-545-5

PROPERTIES

• Suitability: suitable for cell culture
• Suitability: suitable for insect cell culture
• Purity: ≥60% (HPAE)
• Apperance: powder
• Solubility: H2O: soluble50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
• GHS Signal Word: Danger
• GHS Hazard statements: H300 +H310 + H330-H318-H334
• European Hazard Symbols: Highly toxic (T+)
• GHS Precautionary statements: P260-P264-P280-P284-P301 + P310-P302 + P350
• RID/ADR: UN 3462 6.1/PG 1
• Risk Statements: 26/27/28-41-42/43
• RTECS: WK2130000
• Safety Statements: 22-26-28-36/37/39-45
• Storage Temperature: 2-8°C
• Hazard Class: 6.1
• UN Number: 3462
• Packing Group: 1
• German water hazard class: 3

DETAILS

Description (简体中文)

Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation. For use in the selection and maintenance of prokaryotic and eukaryotic cells.
Preparation Note
通过离子交换色谱纯化
Application
Use to study protein synthesis at the level of 70S ribosome translocation and mRNA template misreading. Use as a selection agent for hygromycin resistance gene transformed cells. Recommended for use as a selection agent at 100-800 μg/mL.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information