Home > Compound List > Product Information
Fast Green FCF_Molecular_structure_CAS_2353-45-9)
Click picture or here to close

Fast Green FCF

Catalog No. F7252 Name Sigma Aldrich
CAS Number 2353-45-9 Website http://www.sigmaaldrich.com
M. F. C37H34N2Na2O10S3 Telephone 1-800-521-8956
M. W. 808.8478 Fax
Purity Email
Storage Chembase ID: 109627


IUPAC name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzene-1-sulfonate
IUPAC Traditional name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzenesulfonate
Food green 3


Beilstein Number 5718212
Color Index Number 42053
MDL Number MFCD00013053
PubChem SID 24894934
EC Number 219-091-5
CAS Number 2353-45-9


Compostion Dye content, ≥85%
Melting Point 290 °C (dec.)(lit.)
GHS Pictograms GHS08
GHS Signal Word Warning
GHS Hazard statements H341
European Hazard Symbols Harmful Harmful (Xn)
Personal Protective Equipment Eyeshields, Gloves
GHS Precautionary statements P281
Risk Statements 68
RTECS BQ4425000
Safety Statements 36/37
German water hazard class 2


Description (English)
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.