3,3′,5,5′-Tetramethylbenzidine

• Catalog No.: T5525
• CAS Number: 54827-17-7
• M. F.: C16H20N2
• M. W.: 240.3434

SYNONYMS

• Title: 3,3′,5,5′-四甲基联苯胺
• IUPAC name: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• IUPAC Traditional name: tetramethylbenzidine
• Synonyms: TMB; TMB substrate; Sure Blue TMB; TMB Blotting Plus; BM blue

DATABASE IDS

• CAS Number: 54827-17-7
• Beilstein Number: 2808541
• MDL Number: MFCD00007748
• PubChem SID: 24900293

PROPERTIES

• Apperance: tablet
• Melting Point: 168-171 °C(lit.)
• GHS Signal Word: Warning
• GHS Hazard statements: H319
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS Precautionary statements: P305 + P351 + P338
• Risk Statements: 36
• Safety Statements: 26
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8