(-)-Riboflavin

• Catalog No.: R0508
• CAS Number: 83-88-5
• M. F.: C17H20N4O6
• M. W.: 376.3639

SYNONYMS

• Title: (-)-核黄素
• IUPAC name: 7,8-dimethyl-10-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-2H,3H,4H,10H-benzo[g]pteridine-2,4-dione
• IUPAC Traditional name: riboflavin
• Synonyms: 乳黄素; 维生素 G; 维生素 B2

DATABASE IDS

• PubChem SID: 24899340
• EC Number: 201-507-1
• Beilstein Number: 97825
• CAS Number: 83-88-5
• MDL Number: MFCD00005022

PROPERTIES

• Foreign Activity: Protease, none detected
• Suitability: acrylamide photopolymerization tested
• Melting Point: 290 °C (dec.)(lit.)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• German water hazard class: 1

DETAILS

Description (English)

Application
Riboflavin is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. Riboflavin is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.