Spectinomycin dihydrochloride pentahydrate

• Catalog No.: S9007
• CAS Number: 22189-32-8
• M. F.: C14H36Cl2N2O12
• M. W.: 495.34784

SYNONYMS

• Title: 壮观霉素 二盐酸盐 五水合物
• IUPAC name: (1R,3S,5R,8R,10R,11S,12S,13R,14S)-8,12,14-trihydroxy-5-methyl-11,13-bis(methylamino)-2,4,9-trioxatricyclo[8.4.0.0^3,^8]tetradecan-7-one pentahydrate dihydrochloride
• IUPAC Traditional name: prospec pentahydrate dihydrochloride

DATABASE IDS

• MDL Number: MFCD00150886
• PubChem SID: 24899822
• CAS Number: 22189-32-8
• EC Number: 244-554-3

PROPERTIES

• Potency: ≥603 μg per mg
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C
• German water hazard class: 2

DETAILS

Description (简体中文)

Application
Used as a selection agent for transformed plant cells that contain the selectable marker gene Spcr and to inhibit bacterial protein synthesis at the level of peptidy tRNA translocation.
Biochem/physiol Actions
作用方式：通过干扰肽基 tRNA 的转位抑制蛋白质合成（延伸）。 抗菌谱：革兰氏阴性和革兰氏阳性菌（仅包括淋球菌）。 抗性机理：rpsE（核糖体蛋白 S5 的基因）的突变可阻止壮观霉素的结合。
Spectinomycin is a broad spectrum antibiotic that inhibits protein synthesis (elongation) by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation. Resistance to spectinomycin is conferred by aminoglycoside-3′-adenyltransferase gene (aadA). It is effective against Gram-negative and Gram-positive bacteria. Mutation in rpsE (the gene for ribosomal protein S5) prevents binding of spectinomycin.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information