Fluorescein isothiocyanate isomer I

• Catalog No.: F7250
• CAS Number: 3326-32-7
• M. F.: C21H11NO5S
• M. W.: 389.38074
• Purity: ≥90% (HPLC)

SYNONYMS

• IUPAC name: 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one
• IUPAC Traditional name: FITC
• Synonyms: Fluorescein 5-isothiocyanate; FITC

DATABASE IDS

• CAS Number: 3326-32-7
• EC Number: 222-042-0
• PubChem SID: 24894933
• MDL Number: MFCD00005063
• Beilstein Number: 1407295

PROPERTIES

• Purity: ≥90% (HPLC)
• Suitability: suitable for protein labeling
• Apperance: powder
• Fluorescence: λex 492 nm; λem 518 nm (green)
• Melting Point: >360 °C(lit.)
• GHS Signal Word: Danger
• GHS Hazard statements: H317-H334
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS Precautionary statements: P261-P280-P342 + P311
• Risk Statements: 42/43
• Safety Statements: 22-36/37-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Frequently Asked Questions
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Application
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)