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Fluorescein isothiocyanate isomer I_Molecular_structure_CAS_3326-32-7)
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Fluorescein isothiocyanate isomer I

Catalog No. F7250 Name Sigma Aldrich
CAS Number 3326-32-7 Website http://www.sigmaaldrich.com
M. F. C21H11NO5S Telephone 1-800-521-8956
M. W. 389.38074 Fax
Purity ≥90% (HPLC) Email
Storage Chembase ID: 130829


IUPAC name
IUPAC Traditional name
Fluorescein 5-isothiocyanate


CAS Number 3326-32-7
EC Number 222-042-0
PubChem SID 24894933
MDL Number MFCD00005063
Beilstein Number 1407295


Purity ≥90% (HPLC)
Suitability suitable for protein labeling
Apperance powder
Fluorescence λex 492 nm; λem 518 nm (green)
Melting Point >360 °C(lit.)
GHS Pictograms GHS08
GHS Signal Word Danger
GHS Hazard statements H317-H334
European Hazard Symbols Harmful Harmful (Xn)
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Precautionary statements P261-P280-P342 + P311
Risk Statements 42/43
Safety Statements 22-36/37-45
Storage Temperature 2-8°C
German water hazard class 3


Description (English)
Frequently Asked Questions
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Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)