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DOTAP methosulfate

Catalog No. D1163 Name Sigma Aldrich
CAS Number 144189-73-1 Website http://www.sigmaaldrich.com
M. F. C43H83NO8S Telephone 1-800-521-8956
M. W. 774.18602 Fax
Purity Email
Storage Chembase ID: 132531


IUPAC name
{2,3-bis[(9Z)-octadec-9-enoyloxy]propyl}trimethylazanium methyl sulfate
IUPAC Traditional name
{2,3-bis[(9Z)-octadec-9-enoyloxy]propyl}trimethylazanium methyl sulfate(1-)
Transfection reagent
N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate


MDL Number MFCD00274414
PubChem SID 24893370
CAS Number 144189-73-1


Concentration 1 mg/mL
Apperance clear to hazy colorless to faint yellow buffered aqueous suspension
Personal Protective Equipment Eyeshields, Gloves
Storage Temperature 2-8°C
German water hazard class 3


Description (English)
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
Features and Benefits

• Compatible with both serum and serum-free transfection protocols
• 5-100 times more efficient than calcium phosphate- or DEAE-dextran-based transfection
• Low cytotoxicity results in increased transformation efficiency.
• Compatible for use with in vivo transfection applications
• Packaged to prevent oxidation and peroxide formation, eliminating damage to transfection molecules and cells
Physical form
Suspension in MES-buffered saline, pH 6.2