Riboflavin 5′-monophosphate sodium salt hydrate

• Catalog No.: F1392
• CAS Number: 130-40-5(anhydrous)
• M. F.: C17H22N4NaO10P
• M. W.: 496.340911
• Purity: ≥70%

SYNONYMS

• Title: 核黄素磷酸钠 钠盐 水合物
• IUPAC name: sodium 10-[(2S,3S,4R)-5-(hydrogen phosphonatooxy)-2,3,4-trihydroxypentyl]-7,8-dimethyl-2H,3H,4H,10H-benzo[g]pteridine-2,4-dione hydrate
• IUPAC Traditional name: sodium 10-[(2S,3S,4R)-5-(hydrogen phosphonatooxy)-2,3,4-trihydroxypentyl]-7,8-dimethyl-3H-benzo[g]pteridine-2,4-dione hydrate
• Synonyms: 核黄素-5′-磷酸钠 钠盐; FMN-Na; 黄素单核苷酸

DATABASE IDS

• MDL Number: MFCD00150992
• Beilstein Number: 4106529
• PubChem SID: 24894764
• CAS Number: 130-40-5(anhydrous)
• EC Number: 204-988-6

PROPERTIES

• Foreign Activity: Protease, none detected
• Grade: for electrophoresis
• Purity: ≥70%
• Suitability: suitable for acrylamide photopolymerization
• Absorption Wavelength: ε1mM/445 nm 12.5
• Apperance: powder
• Optical Rotation: [α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
FMN is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, TEMED or DMAPN, are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.