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Riboflavin 5′-monophosphate sodium salt hydrate_Molecular_structure_CAS_130-40-5(anhydrous))
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Riboflavin 5′-monophosphate sodium salt hydrate

Catalog No. F1392 Name Sigma Aldrich
CAS Number 130-40-5(anhydrous) Website http://www.sigmaaldrich.com
M. F. C17H22N4NaO10P Telephone 1-800-521-8956
M. W. 496.340911 Fax
Purity ≥70% Email
Storage Chembase ID: 130811

SYNONYMS

Title
核黄素磷酸钠 钠盐 水合物
IUPAC name
sodium 10-[(2S,3S,4R)-5-(hydrogen phosphonatooxy)-2,3,4-trihydroxypentyl]-7,8-dimethyl-2H,3H,4H,10H-benzo[g]pteridine-2,4-dione hydrate
IUPAC Traditional name
sodium 10-[(2S,3S,4R)-5-(hydrogen phosphonatooxy)-2,3,4-trihydroxypentyl]-7,8-dimethyl-3H-benzo[g]pteridine-2,4-dione hydrate
Synonyms
核黄素-5′-磷酸钠 钠盐
FMN-Na
黄素单核苷酸

DATABASE IDS

MDL Number MFCD00150992
Beilstein Number 4106529
PubChem SID 24894764
CAS Number 130-40-5(anhydrous)
EC Number 204-988-6

PROPERTIES

Foreign Activity Protease, none detected
Grade for electrophoresis
Purity ≥70%
Suitability suitable for acrylamide photopolymerization
Absorption Wavelength ε1mM/445 nm 12.5
Apperance powder
Optical Rotation [α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Storage Temperature -20°C
German water hazard class 3

DETAILS

Description (English)
Application
FMN is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, TEMED or DMAPN, are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

REFERENCES