5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

• Catalog No.: B6777
• CAS Number: 6578-06-9
• M. F.: C15H15BrClN2O4P
• M. W.: 433.621361
• Purity: ≥99%

SYNONYMS

• Title: 5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐
• IUPAC name: 4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)oxy]phosphonic acid
• IUPAC Traditional name: (5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine
• Synonyms: BCIP® 对甲苯胺盐; X-phosphate p-toluidine salt

DATABASE IDS

• PubChem SID: 24891823
• EC Number: 229-506-1
• MDL Number: MFCD00040636
• CAS Number: 6578-06-9

PROPERTIES

• Foreign Activity: Protease, none detected
• Grade: for molecular biology
• Purity: ≥99%
• Apperance: powder
• Solubility: DMF: soluble20 mg/mL
• Solubility: H2O: insoluble
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC