Aminopterin

• Catalog No.: A5159
• CAS Number: 54-62-6
• M. F.: C19H20N8O5
• M. W.: 440.4127

SYNONYMS

• Title: 氨基蝶呤
• IUPAC name: (2S)-2-[(4-{[(2,4-diaminopteridin-6-yl)methyl]amino}phenyl)formamido]pentanedioic acid
• IUPAC Traditional name: 4-aminopteroylglutamic acid
• Synonyms: 4-氨基叶酸; 4-氨基-PGA; N-[4-[[(2,4-二氨基-6-蝶啶)甲基]氨基]苯甲酰]-L-谷氨酸

DATABASE IDS

• CAS Number: 54-62-6
• Beilstein Number: 69045
• EC Number: 200-209-9
• PubChem SID: 24890950
• MDL Number: MFCD00036692

PROPERTIES

• Concentration: 50 ×
• Grade: Hybri-Max™
• Impurities: endotoxin, tested
• Shipped in: dry ice
• Sterility: γ-irradiated
• Suitability: suitable for hybridoma
• Gene Information: human ... FPGS(2356)mouse ... Fpgs(14287)
• Absorption Wavelength: ε1M/261 nm, 0.1 M NaOH 25,700
• Absorption Wavelength: ε1M/282 nm, 0.1 M NaOH 24,500
• Absorption Wavelength: ε1M/373 nm, 0.1 M NaOH 8,100
• Apperance: lyophilized powder
• GHS Signal Word: Danger
• GHS Hazard statements: H300-H360
• European Hazard Symbols: Highly toxic (T+)
• Personal Protective Equipment: Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
• GHS Precautionary statements: P201-P264-P301 + P310-P308 + P313
• RID/ADR: UN 2811 6.1/PG 1
• Risk Statements: 61-28
• RTECS: MA1050000
• Safety Statements: 53-28-36/37-45
• Storage Temperature: -20°C
• Hazard Class: 6.1
• UN Number: 2811
• Packing Group: 1
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
用于杂交瘤细胞培养应用的氨基蝶呤浓缩物添加剂。
Linkage
比甲氨喋呤更有效，但毒性也更强。
Reconstitution
10mL 无菌细胞培养基可复原一小瓶本品。储备液足以制备 500mL 培养基。最终工作浓度：0.4μM 氨基蝶呤。
Biochem/physiol Actions
叶酸拮抗剂。氨基蝶呤是由叶酸转运蛋白运输至细胞内的。在细胞内，它被叶酰聚谷氨酸合酶转化为一种高分子量的聚谷氨酸盐代谢物，该产物又与二氢叶酸还原酶结合并抑制其活性。氨基蝶呤-聚谷氨酸盐经 γ-谷氨酰基水解酶作用发生细胞内降解。
法律信息
Hybri-Max 商标 Sigma-Aldrich Co. LLC
Other Notes
Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. A5159.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.