Brilliant Blue R Staining Solution

• Catalog No.: B6529
• CAS Number: 6104-59-2
• M. F.: C45H44N3NaO7S2
• M. W.: 825.96653

SYNONYMS

• IUPAC name: sodium 3-({[4-({4-[(4-ethoxyphenyl)amino]phenyl}(4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene)methyl)phenyl](ethyl)amino}methyl)benzene-1-sulfonate
• IUPAC Traditional name: sodium 3-({[4-({4-[(4-ethoxyphenyl)amino]phenyl}(4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene)methyl)phenyl](ethyl)amino}methyl)benzenesulfonate
• Synonyms: Brilliant indocyanin 6B; Acid Blue 83; Coomassie Brilliant Blue R; Brilliant Blue R

DATABASE IDS

• Color Index Number: 42660
• Beilstein Number: 5718025
• PubChem SID: 24891914
• CAS Number: 6104-59-2
• MDL Number: MFCD00041762

PROPERTIES

• Apperance: ethanol solution
• Flash Point: 27 °C
• Flash Point: 80.6 °F
• GHS Signal Word: Warning
• GHS Hazard statements: H226-H315-H319
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P305 + P351 + P338
• RID/ADR: UN 1170 3/PG 3
• Risk Statements: 10-36/38
• Safety Statements: 26
• Hazard Class: 3
• UN Number: 1170
• Packing Group: 3
• German water hazard class: 2

DETAILS

Description (English)

Components
0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.
Analysis Note
Tested for suitability on agarose gels following immunoelectrophoresis.
Application
Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.
For detection of protein bands following electrophoresis.