Isopropyl β-D-1-thiogalactopyranoside

• Catalog No.: I5502
• CAS Number: 367-93-1
• M. F.: C9H18O5S
• M. W.: 238.30122
• Purity: ≥99% (TLC)

SYNONYMS

• Title: 异丙基 β-D-1-硫代半乳糖吡喃糖苷
• IUPAC name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
• IUPAC Traditional name: IPTG
• Synonyms: IPTG; 异丙基 β-D-硫代半乳糖苷

DATABASE IDS

• CAS Number: 367-93-1
• PubChem SID: 24896069
• MDL Number: MFCD00063273
• EC Number: 206-703-0
• Beilstein Number: 4631

PROPERTIES

• Description: non-ionic
• Impurities: 10.0-22.0% Dioxane
• Purity: ≥99% (TLC)
• Quality Level: PREMIUM
• GHS Signal Word: Warning
• GHS Hazard statements: H319-H351
• European Hazard Symbols: Harmful (Xn)
• GHS Precautionary statements: P281-P305 + P351 + P338
• Risk Statements: 19-40
• Safety Statements: 36/37
• Storage Temperature: 2-8°C
• Supplemental Hazard Statements: May form explosive peroxides.
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。
Other Notes
非代谢性半乳糖类似物。
Reconstitution
将 IPTG 溶于水，然后经无菌过滤，配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。