Fast Green FCF

• Catalog No.: F7258
• CAS Number: 2353-45-9
• M. F.: C37H34N2Na2O10S3
• M. W.: 808.8478

SYNONYMS

• IUPAC name: disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzene-1-sulfonate
• IUPAC Traditional name: disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzenesulfonate
• Synonyms: Food green 3

DATABASE IDS

• Beilstein Number: 5718212
• Color Index Number: 42053
• MDL Number: MFCD00013053
• EC Number: 219-091-5
• CAS Number: 2353-45-9
• PubChem SID: 24894936

PROPERTIES

• Personal Protective Equipment: Eyeshields, Gloves
• GHS Precautionary statements: P281
• Risk Statements: 68
• RTECS: BQ4425000
• Safety Statements: 36/37
• German water hazard class: 2
• GHS Signal Word: Warning
• GHS Hazard statements: H341
• European Hazard Symbols: Harmful (Xn)
• Grade: certified by the Biological Stain Commission
• Melting Point: 290 °C (dec.)(lit.)

DETAILS

Description (English)

Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Suitability
Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.