DL-Dithiothreitol

• Catalog No.: D5545
• CAS Number: 3483-12-3
• M. F.: C4H10O2S2
• M. W.: 154.251
• Purity: ≥99.0% (titration)

SYNONYMS

• Title: DL-二硫苏糖醇
• IUPAC name: (2S,3S)-1,4-disulfanylbutane-2,3-diol
• IUPAC Traditional name: dithiothreitol
• Synonyms: 克莱兰氏试剂; DTT; 1,4-二巯基苏糖醇

DATABASE IDS

• MDL Number: MFCD00004877
• EC Number: 222-468-7
• CAS Number: 3483-12-3
• Beilstein Number: 1719757
• PubChem SID: 24893941

PROPERTIES

• Antion Traces: sulfate (SO42-): ≤0.005%
• Cation Traces: Al: ≤0.0005%
• Cation Traces: As: ≤0.0001%
• Cation Traces: Ba: ≤0.0005%
• Cation Traces: Bi: ≤0.0005%
• Cation Traces: Ca: ≤0.001%
• Cation Traces: Cd: ≤0.0005%
• Cation Traces: Co: ≤0.0005%
• Cation Traces: Cr: ≤0.0005%
• Cation Traces: Cu: ≤0.0005%
• Cation Traces: Fe: ≤0.0005%
• Cation Traces: K: ≤0.005%
• Cation Traces: Li: ≤0.0005%
• Cation Traces: Mg: ≤0.0005%
• Cation Traces: Mn: ≤0.0005%
• Cation Traces: Mo: ≤0.0005%
• Cation Traces: Na: ≤0.1%
• Cation Traces: Ni: ≤0.0005%
• Cation Traces: Pb: ≤0.0005%
• Cation Traces: Sr: ≤0.0005%
• Cation Traces: Zn: ≤0.0005%
• Impurities: Insoluble matter, passes filter test
• Impurities: ≤0.3% 4,5-dihydroxy-1,2-dithiane
• Purity: ≥99.0% (titration)
• Quality Level: PREMIUM
• Absorption Wavelength: A0.1M/260, H2O ≤0.400
• Absorption Wavelength: A0.1M/280, H2O ≤0.100
• Flash Point: 113 °C
• Flash Point: 235.4 °F
• Melting Point: 41-44 °C(lit.)
• pH: 4.0-6.0 (20-25 °C, 0.1 m in H2O)
• Solubility: H2O: soluble0.1 M, clear, colorless
• GHS Signal Word: Warning
• GHS Hazard statements: H302-H315-H319-H335
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 22-36/37/38
• RTECS: EK1610000
• Safety Statements: 26-36
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Application
用于将 -SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。