MCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(DNP)-D-Arg-NH2

• Catalog No.: 03MET011
• M. F.: C84H120N22O19
• M. W.: 1741.9876

SYNONYMS

• IUPAC name: (2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S,3S)-2-{[(2S)-1-[(2S)-6-amino-2-{2-[(7-methoxy-2-oxo-2H-chromen-4-yl)formamido]acetamido}hexanoyl]pyrrolidin-2-yl]formamido}-3-methylpentanamido]-4-methylpentanamido]-3-phenylpropanamido]-3-phenylpropanamido]-5-carbamimidamidopentanamido]-4-methylpentanamido]-N-[(1R)-4-carbamimidamido-1-carbamoylbutyl]-6-[(2,4-dinitrophenyl)amino]hexanamide
• IUPAC Traditional name: (2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S,3S)-2-{[(2S)-1-[(2S)-6-amino-2-{2-[(7-methoxy-2-oxochromen-4-yl)formamido]acetamido}hexanoyl]pyrrolidin-2-yl]formamido}-3-methylpentanamido]-4-methylpentanamido]-3-phenylpropanamido]-3-phenylpropanamido]-5-carbamimidamidopentanamido]-4-methylpentanamido]-N-[(1R)-4-carbamimidamido-1-carbamoylbutyl]-6-[(2,4-dinitrophenyl)amino]hexanamide
• Synonyms: MCA-GKPILFFRLL(DNP)DR-NH2; MCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(DNP)-D-Arg-NH2

DETAILS

Description (English)

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (K_cat/K_m = 15.6 μM^-1s^-1 and 10.9 μM^-1s^-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates. MCA = (7-Methoxycoumarin-4-yl)acetyl
Excitation max.: ~328 nm, Emission max.: ~393 nm

REFERENCES

• Yasuda, Y et al., J. Biochem., 125:1137 (1999).