Z-Asp-Glu-Val-Asp-7-Amino-4-trifluoromethylcoumarin

• Catalog No.: 03AFC138Z
• M. F.: C36H38F3N5O14
• M. W.: 821.7072296

SYNONYMS

• IUPAC name: (4S)-4-[(2S)-2-{[(benzyloxy)carbonyl]amino}-3-carboxypropanamido]-4-{[(1S)-1-{[(1S)-2-carboxy-1-{[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]carbamoyl}ethyl]carbamoyl}-2-methylpropyl]carbamoyl}butanoic acid
• IUPAC Traditional name: (4S)-4-[(2S)-2-{[(benzyloxy)carbonyl]amino}-3-carboxypropanamido]-4-{[(1S)-1-{[(1S)-2-carboxy-1-{[2-oxo-4-(trifluoromethyl)chromen-7-yl]carbamoyl}ethyl]carbamoyl}-2-methylpropyl]carbamoyl}butanoic acid
• Synonyms: Z-Asp-Glu-Val-Asp-AFC; Z-DEVD-AFC

DETAILS

Description (English)

A fluorogenic substrate for Caspase-3 (CPP32, YAMA). Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Excitation max.: ~400 nm, Emission max.: ~505 nm

REFERENCES

• Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
• Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
• Kluck, R.M., et al. 1997. Science 275, 1132.
• Talanian, J., et al. 1997. J. Biol. Chem. 272, 9677.
• Chinnaiyan, A.M., et al. 1996. Curr. Biol. 6, 897.
• Nicholson, D.W., et al. 1995. Nature 376, 37.