4-{bis[4-(dimethylamino)phenyl]methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride

• ChemBase ID: 289
• Molecular Formular: C25H30ClN3
• Molecular Mass: 407.9788
• Monoisotopic Mass: 407.21282566
• SMILES: N(c1ccc(/C(=C/2\C=CC(=[N+](C)C)C=C2)/c2ccc(N(C)C)cc2)cc1)(C)C.[Cl-]
• Canonical SMILES: C[N+](=C1C=C/C(=C(/c2ccc(cc2)N(C)C)\c2ccc(cc2)N(C)C)/C=C1)C.[Cl-]
• InChI: InChI=1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChIKey: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

NAMES AND DATABASE IDS

Names

• IUPAC name: 4-{bis[4-(dimethylamino)phenyl]methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride
• IUPAC Traditional name: gentian violet chloride
• Brand Name: Hecto Violet R; Hectograph Violet SR; Hexamethyl Violet; Hexamethylpararosaniline chloride; Hidaco Brilliant Crystal Violet; Hidaco crystal Violet; Meroxyl-Wander; Meroxylan-Wander; Methyl Violet 10B; Methyl Violet 10BD; Methyl Violet 10BK; Methyl Violet 10BN; Methyl Violet 10BNS; Methyl Violet 10BO; Methyl Violet 5BNO; Methyl Violet 5BO; Methyl Violet 6B; Methyl violet; Methylrosaniline chloride; Mitsui Crystal Violet; Oxiuran; Oxycolor; Oxyozyl; Paper Blue R; Plastoresin Violet 5BO; Pyoktanin; Vermicid; Vianin; Viocid; Violet 5BO; Violet 6BN; Violet CP; Violet XXIII; crystal violet for microscopy; gentian violet gr; gention violet alchoholic staining solution; methylrosanilinium chloride; Adergon; Aizen Crystal Violet; Aizen Crystal Violet Extra Pure; Aniline Violet; Aniline Violet pyoktanine; Atmonil; Avermin; Axuris; Badil; Basic Violet 3; Basic Violet BN; Basic Violet-3; Bismuth Violet; Brilliant Violet 5B; C.I. Basic Violet 3; Calcozine Violet 6BN; Calcozine Violet C; Crystal Violet 10B; Crystal Violet 5BO; Crystal Violet 6B; Crystal Violet 6BO; Crystal Violet AO; Crystal Violet AON; Crystal Violet BP; Crystal Violet BPC; Crystal Violet Extra Pure; Crystal Violet Extra Pure APN; Crystal Violet Extra Pure APNX; Crystal Violet FN; Crystal Violet HL2; Crystal Violet O; Crystal Violet Pure DSC; Crystal Violet Pure DSC Brilliant; Crystal Violet SS; Crystal Violet Technical; Crystal Violet USP; Crystal Violet base; Crystal Violet chloride; Crystal Violet chloride salt; GV-Eleven; Genapax; Gentersal; Gentiaverm; Genticid; Gentioletten; Gvs
• Synonyms: BRILLIANT VIOLET; Crystal Violet; Crystal Violet; Gentian Violet; Gentian violet; Crystal violet; Basic Violet 3; Hexamethylpararosaniline chloride; Methyl Violet 10B; N-[4-[Bis[4-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-methylmethanaminium Chloride; Adergon; Crystal Violet Chloride; Gentersal; Meroxyl; Oxiuran; Oxycolor; Oxyozyl; Violet CP; Violet XXIII; C.I. 42555; Crystal Violet, ACS; Gentian Violet; Gentian Violet B; Methyl violet; CARBOL CRYSTAL VIOLET; HEXAMETHYL VIOLET; CARBOL GENTIAN VIOLET; GENTIAN VIOLET, ACS; Methylrosaniline chloride; Gentian Violet solution; Gram-Hucker’s stain solution (I); Gentian Violet solution; Crystal violet solution; 结晶紫; 结晶紫, ACS; 六甲副蔷薇苯胺氯化物; 甲基紫 10B; 碱性紫 3; 结晶紫; 龙胆紫; 龙胆紫; Gram-Hucker’s stain solution (I); 龙胆紫 溶液; 结晶紫 溶液

Database IDs

• CAS Number: 14426-25-6; 548-62-9; 8004-87-3; 39393-39-0
• EC Number: 208-953-6
• MDL Number: MFCD00011750
• Beilstein Number: 3580948; 4077708; 8464808
• Merck Index: 144395
• PubChem SID: 24892651; 24872296; 24882056; 24892842; 24895083; 46505353; 24892272; 24859768; 24895895; 24870082; 24859619; 160963752
• PubChem CID: 3468; 196986; 11057
• CHEMBL: 64894
• Chemspider ID: 170606
• Wikipedia Title: Methyl_violet
• Color Index Number: 42555

CALCULATED PROPERTIES

JChem

• H Acceptors: 2
• H Donor: 0
• LogD (pH = 5.5): 1.3081571
• LogD (pH = 7.4): 1.3943535
• Log P: 1.3955112
• Molar Refractivity: 146.0039 cm^3
• Polarizability: 45.870586 Å^3
• Polar Surface Area: 9.49 Å^2
• Rotatable Bonds: 4
• Lipinski's Rule of Five: true

ALOGPS 2.1

• Log P: 0.87
• LOG S: -5.32
• Solubility (Water): 1.93e-03 g/l

PROPERTIES

Physical Property

• Solubility: 4 mg/mL; Chloroform; DMSO; Soluble in water, ethanol, insoluble in xylene
• Apperance: Dark Green Solid; Green to dark-green powder; Powder; S. No.: 785
• Melting Point: 137 °C (279 °F) – decomposes; 205 °C (dec.)(lit.); 208-210°C; 215°C; ca 215°C dec.
• Flash Point: 37 °C; 98.6 °F
• Absorption Wavelength: / passes test; λmax 590 nm
• Hydrophobicity(logP): 3.18

Safety Information

• Storage Condition: -20°C; Amber Vial, Refrigerator; Room Temperature (15-30°C); Room Temperature (15-30°C), Protect from light
• RTECS: BO9000000
• European Hazard Symbols: Corrosive (C); Nature polluting (N); Toxic (T); X; Harmful (Xn)
• UN Number: 1993; 2790; 3077; UN3077
• German water hazard class: 2; 3
• Hazard Class: 3; 8; 9
• Packing Group: 2; 3; III
• Risk Statements: 10-40-51/53; 22-40-41-50/53; 34-51/53; 45-22-41-50/53-68; 51/53; R:22-40
• Safety Statements: 26-36/37/39-45-61; 26-36/37/39-46-60-61; 36/37-61; 53-26-36/37/39-45-61; 61; S:36/37/39
• TSCA Listed: 是
• GHS Pictograms: GHS02; GHS05; GHS07; GHS08; GHS09
• GHS Signal Word: Danger; Warning
• GHS Hazard statements: H226-H319-H351-H412; H302-H315-H318-H341-H350-H410; H302-H315-H318-H351-H410; H302-H318-H351-H410; H314-H411; H318-H351-H400-H410-H302; H411
• GHS Precautionary statements: P201-P273-P280-P305 + P351 + P338-P308 + P313-P501; P273; P273-P280-P305 + P351 + P338-P310; P273-P280-P305 + P351 + P338-P501; P273-P281-P305 + P351 + P338; P280-P281-P305+P351+P338-P310-P405-P501A
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves; Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges; Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US); Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
• RID/ADR: UN 1993 3/PG 3; UN 2790 8/PG 2; UN 3077 9/PG 3

Product Information

• Purity: ≥90.0% anhydrous basis; 90+%; Dye Content ~95%
• Concentration: 0.5% in anhydrous acetic acid
• Grade: ACS; ACS reagent; certified by the Biological Stain Commission; for microscopy; for microscopy (Bact., Bot., Hist., Vit.); for microscopy (Bact., Hist.); indicator (pH 0.1-2.0); indicator for non-aqueous titration; indicator for the determination of the redox potential; JIS special grade; REAGENT; SAJ first grade; SAJ special grade; VETRANAL™, analytical standard
• Salt Data: Chlorate
• Compostion: Dye content, ≥90%; Gentian Violet, 1 % (w/v)
• Suitability: complies for sensitivity; meets USP testing specifications; passes test for sensitivity as indicator
• Clinical: for in vitro diagnostic use
• Shelf Life: (Expiry date on the label); (limited shelf life, expiry date on the label)
• Loss on Drying: ≤2.5% loss on drying; ≤7.5% loss on drying
• Quality Level: IVD
• Linear Formula: C25H30N3Cl
• Empirical Formula (Hill Notation): C25H30ClN3

DETAILS

MP Biomedicals - 05213025

MP Biomedicals Rare Chemical collection

MP Biomedicals - 02152511

ACS Reagent Grade
Assay ≥90.0% anhydrous dye

MP Biomedicals - 05208589

MP Biomedicals Rare Chemical collection

MP Biomedicals - 05207551

MP Biomedicals Rare Chemical collection

MP Biomedicals - 05207018

MP Biomedicals Rare Chemical collection

MP Biomedicals - 02101775

Dye content: ~ 95%

DrugBank - DB00406

• Drug Groups: approved
• Description: A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties. [PubChem]
• Indication: For the treatment of bacterial and fungal infections inside the mouth (thrush) and skin, also for the prevention of transmission of Chagas' disease (as a blood additive).
• Pharmacology: Gentian violet is a mutagen, a mitotic poison, and a clastogen. Gentian violet has been used in medicine for almost 100 years: as an antiseptic for external use, as a topical antibiotic, as a topical antifungal agent, as an antihelminthic agent by oral administration, and more recently, as a blood additive to prevent transmission of Chagas' disease. It is thought to work by binding to the DNA of target organisms and causing disruption, mutation or inhibition of DNA replication.
• Toxicity: LD₅₀=420 mg/kg (rat, oral). Oral administration can cause gastrointestinal irritation, and intravenous injection can cause depression in the white blood cell count.
• Affected Organisms: Yeast and other fungi; Bacteria and protozoa
• Biotransformation: Primarily hepatic, mostly demethylation
• External Links: Wikipedia; Drugs.com

Selleck Chemicals - S1917

Research Area: Infection
Biological Activity:
Crystal violet is a triarylmethane dye. The dye is used as a histological stain and in Gram’s method of classifying bacteria. Crystal violet has antibacterial, antifungal and anthelmintic properties,which was formerly important as a topical antiseptic. [1]

Sigma Aldrich - 48770

General description
mixture of crystal violet [C.I.No. 42555] and methyl violet [C.I.No. 42535]
Other Notes
Dye employed for preparing Gram stain1

Sigma Aldrich - HT901

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Components
HT901 is composed of certified crystal violet, 2.3%, ammonium oxalate, 0.1%, and 20% ethyl alcohol, SD3A.
Application
Kit component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).

Sigma Aldrich - C6158

包装
50, 100 g in glass bottle

Sigma Aldrich - C0775

Application
Used as the active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-postive bacteria.
包装
25, 100 g in glass bottle
Suitability
Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Sigma Aldrich - C3886

Application
Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

Sigma Aldrich - HT90132

Components
Contains 2.3% certified crystal violet, 0.1% ammonium oxalate and 20% ethyl alcohol, SD3A.
Application
Intended for use in the Gram Stain Procedure No. HT90.

Sigma Aldrich - 46364

Legal Information
VETRANAL is a trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - G2039

Application
Gentian Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

Sigma Aldrich - 49144

Components
Composition : Gentian violet 5.0 g/l in water
General description
Gentian violet is used in the Gram staining for distinguishing between gram-positive and gram-negative organisms.

Sigma Aldrich - 229288

Packaging
25, 100 g in glass bottle

Sigma Aldrich - 12-1521

Components
Composition : Crystal violet in water

Sigma Aldrich - 32909

General description
Visit our Titration Center to learn more.

Sigma Aldrich - 12-0330

Suitability
bacterial staining

Toronto Research Chemicals - G362500

Anti-infective (topical). Has been used as anthelmintic (Nematodes).

REFERENCES

• http://en.wikipedia.org/wiki/Crystal_violet
• Hodge, H.C., et al.: Toxicol. Appl. Pharmacol., 22, 1 (1972)
• Docampo, R., et al.: Drug Metab. Rev., 22, 161 (1972)