2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol

• ChemBase ID: 155574
• Molecular Formular: C14H27N3O11
• Molecular Mass: 413.37768
• Monoisotopic Mass: 413.1645587
• SMILES: C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
• Canonical SMILES: OC(=O)CN(CC(=O)O)CCN(CC(=O)O)CC(=O)O.OCC(CO)(CO)N
• InChI: InChI=1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• InChIKey: VLEIUWBSEKKKFX-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol
• IUPAC Traditional name: edta; tris buffer
• Synonyms: TRIS--EDTA solution; TE buffer solution; Tris-EDTA buffer solution; TE 缓冲液 溶液

Database IDs

• EC Number: 231-791-2
• MDL Number: MFCD00236359
• PubChem SID: 24888617; 162249712
• PubChem CID: 16218629

CALCULATED PROPERTIES

• Acid pKa: 1.486185
• H Acceptors: 10
• H Donor: 4
• LogD (pH = 5.5): -10.586659
• LogD (pH = 7.4): -14.721412
• Log P: -5.221795
• Molar Refractivity: 62.3456 cm^3
• Polarizability: 24.69443 Å^3
• Polar Surface Area: 155.68 Å^2
• Rotatable Bonds: 14
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Density: 1.050-1.070 g/mL at 20 °C
• Refractive Index: n20/D 1.352-1.354; n20/D 1.360
• Absorption Wavelength: λ: 260 nm Amax: 0.03; λ: 260 nm Amax: 0.04; λ: 280 nm Amax: 0.01; λ: 280 nm Amax: 0.05
• pH: 7.4±0.2 (25 °C); 8.0±0.1 (25 °C); 8.8-9.2 (25 °C)

Safety Information

• European Hazard Symbols: Irritant (Xi)
• German water hazard class: 1; 2; nwg
• Risk Statements: 41
• Safety Statements: 26-39
• GHS Pictograms: GHS05
• GHS Signal Word: Danger
• GHS Hazard statements: H318
• GHS Precautionary statements: P280-P305 + P351 + P338
• Personal Protective Equipment: Eyeshields, Gloves

Product Information

• Concentration: 0.23-0.27 M; 100 ×
• Grade: for molecular biology
• Impurities: DNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• Cation Traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• Foreign Activity: DNase, RNase, none detected
• Sterility: 0.2 μm filtered
• Quality: 100× concentrate
• λ: neat
• Product Line: BioUltra
• abs.: absorption/260 nm cut-off

DETAILS

Sigma Aldrich - T9285

Frequently Asked Questions
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Other Notes
1.0 M Tris-HCl, pH approx. 8.0, containing 0.1 M EDTA

Sigma Aldrich - 86377

Physical form
1.0 M TRIS HCl, pH 8.0±0.1 (25°C); 0.1 M EDTA.Na2

Sigma Aldrich - 98306

Physical form
0.25 M EDTA sodium salt; 0.25 M TRIS hydrochloride in water

Sigma Aldrich - 93302

Physical form
10 mM Tris-HCl; 1 mM EDTA

Sigma Aldrich - 93283

Other Notes
This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.