(2S)-6-amino-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-6-amino-2-[(2S)-2-[(2S)-2-amino-3-carboxypropanamido]-3-(4-hydroxyphenyl)propanamido]hexanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]hexanoic acid

• ChemBase ID: 133316
• Molecular Formular: C41H60N10O20
• Molecular Mass: 1012.9701
• Monoisotopic Mass: 1012.39853437
• SMILES: c1cc(ccc1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O
• Canonical SMILES: NCCCC[C@@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)O)CCCCN)CC(=O)O)CC(=O)O)CC(=O)O)CC(=O)O)NC(=O)[C@@H](NC(=O)[C@H](CC(=O)O)N)Cc1ccc(cc1)O
• InChI: InChI=1S/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/m0/s1
• InChIKey: XZWYTXMRWQJBGX-VXBMVYAYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: (2S)-6-amino-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-6-amino-2-[(2S)-2-[(2S)-2-amino-3-carboxypropanamido]-3-(4-hydroxyphenyl)propanamido]hexanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]hexanoic acid
• IUPAC Traditional name: (2S)-6-amino-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-2-[(2S)-6-amino-2-[(2S)-2-[(2S)-2-amino-3-carboxypropanamido]-3-(4-hydroxyphenyl)propanamido]hexanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]-3-carboxypropanamido]hexanoic acid
• Synonyms: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys; ddddk peptide; dykddddk peptide; FLAG® Peptide

Database IDs

• MDL Number: MFCD02262135
• PubChem SID: 162227593
• PubChem CID: 9854670

CALCULATED PROPERTIES

• Acid pKa: 2.6215737
• H Acceptors: 23
• H Donor: 17
• LogD (pH = 5.5): -17.942945
• LogD (pH = 7.4): -24.21316
• Log P: -11.397852
• Molar Refractivity: 232.9804 cm^3
• Polarizability: 92.33325 Å^3
• Polar Surface Area: 525.79 Å^2
• Rotatable Bonds: 35
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Apperance: lyophilized powder

Safety Information

• German water hazard class: 3
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C

Product Information

• Shipped in: wet ice
• Mol. Weight: mol wt 1012.97 Da

DETAILS

Sigma Aldrich - F3290

Application
Gently elutes FLAG fusion proteins from ANTI-FLAG® M1 and M2 affinity resins. Usual working concentration is 100 μg/ml. Will not elute 3X FLAG fusion proteins. For this application, use 3X FLAG Peptide, F4799.Browse additional application references in our FLAG® Literature portal.
Preparation Note
For stock solution, dissolve in TBS (10mM Tris HCL, 150 mM NaCl, pH7.4) to a final concentration of 5 mg/mL.
General description
The FLAG peptide is used for the competitive elution of amino-terminal, Met-amino-terminal or carboxy-terminal FLAG fusion proteins from the Anti-FLAG M1 or Anti-FLAG M2 antibody (in solution or bound to an agarose gel).
法律信息
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC