bis(3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine) dichlorozinc dihydrochloride

• ChemBase ID: 132355
• Molecular Formular: C34H40Cl4N6Zn
• Molecular Mass: 739.9136
• Monoisotopic Mass: 736.13599803
• SMILES: CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.Cl.Cl.Cl[Zn]Cl
• Canonical SMILES: CN(c1ccc2c(c1)nc1c(c2)ccc(c1)N(C)C)C.CN(c1ccc2c(c1)nc1c(c2)ccc(c1)N(C)C)C.Cl[Zn]Cl.Cl.Cl
• InChI: InChI=1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• InChIKey: RAHGLSRJKRXOSY-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: bis(3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine) dichlorozinc dihydrochloride
• IUPAC Traditional name: bis(acridine orange) zinc chloride dihydrochloride
• Synonyms: 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt; 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt); Basic Orange 14; Acridine Orange hemi(zinc chloride) salt

Database IDs

• CAS Number: 10127-02-3
• EC Number: 233-353-6
• MDL Number: MFCD00081043
• Beilstein Number: 3734978
• PubChem SID: 162226632; 24891048; 24849765
• PubChem CID: 16211342
• Color Index Number: 46005

CALCULATED PROPERTIES

• H Acceptors: 3
• H Donor: 0
• LogD (pH = 5.5): 2.0666623
• LogD (pH = 7.4): 2.9319835
• Log P: 3.7223153
• Molar Refractivity: 84.9147 cm^3
• Polarizability: 34.26807 Å^3
• Polar Surface Area: 19.37 Å^2
• Rotatable Bonds: 4
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Absorption Wavelength: λmax 489 nm

Safety Information

• German water hazard class: 3
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

Product Information

• Grade: for microscopy (Fl., Hist.); for molecular biology
• Compostion: Dye content, ~80%; Dye content, 90%
• Linear Formula: C17H20ClN3 · HCl · 1/2ZnCl2

DETAILS

Sigma Aldrich - A6014

Application
Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of DNA in apoptosis studies.
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
包装
10, 25, 100 g in glass bottle

Sigma Aldrich - 01660

Other Notes
Versatile probe of nucleic acids and other cell constituents1
Application
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Sigma Aldrich - 158550

Application
Biological stain
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Packaging
10, 25, 100 g in glass bottle