disodium hydrogen phosphate

• ChemBase ID: 130837
• Molecular Formular: HNa2O4P
• Molecular Mass: 141.958841
• Monoisotopic Mass: 141.9407837
• SMILES: OP(=O)([O-])[O-].[Na+].[Na+]
• Canonical SMILES: [O-]P(=O)(O)[O-].[Na+].[Na+]
• InChI: InChI=1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
• InChIKey: BNIILDVGGAEEIG-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium hydrogen phosphate
• IUPAC Traditional name: disodium hydrogen phosphate
• Synonyms: Sodium hydrogen phosphate, anhydrous, ACS; Disodium hydrogen phosphate solution; di-Sodium hydrogen phosphate solution; Sodium phosphate dibasic solution; Phosphate Standard for IC; sec-Sodium phosphate; Disodium hydrogen phosphate; Disodium phosphate; Sodium hydrogenphosphate; Sodium phosphate dibasic; Sodium phosphate dibasic solution; Phosphate, certified anion standard solution; Dinatrii phosphas anhydricus; Sodium phosphate, dibasic; Sodium hydrogen phosphate; 无水磷酸氢二钠, ACS; 二盐基性磷酸钠 溶液; 磷酸二钠 溶液; 磷酸氢二钠 溶液; 磷酸根 IC 标准溶液; 二盐基磷酸钠; 磷酸一氢钠; 磷酸二钠; 磷酸氢二钠; 磷酸钠 二元; 磷酸氢二钠 溶液; 磷酸根离子，认证阴离子标准液 溶液; 磷酸氢二钠

Database IDs

• CAS Number: 7558-79-4
• EC Number: 231-448-7; 231-791-2
• MDL Number: MFCD00003496
• Merck Index: 148659
• PubChem SID: 24899559; 24901046; 24899638; 24886240; 24845716; 24899856; 24889758; 24855481; 24887682; 24886237; 24899552; 24899448; 162225115; 24858358; 24899769
• PubChem CID: 24203
• FEMA ID: 2399

CALCULATED PROPERTIES

• Acid pKa: 1.7961261
• H Acceptors: 4
• H Donor: 1
• LogD (pH = 5.5): -3.3916447
• LogD (pH = 7.4): -3.9718332
• Log P: -1.0201038
• Molar Refractivity: 12.4085 cm^3
• Polarizability: 5.685633 Å^3
• Polar Surface Area: 83.42 Å^2
• Rotatable Bonds: 0
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble0.5 M at 20 °C, clear, colorless; H2O: soluble1 M at 20 °C, clear, colorless; H2O: soluble100 mg/mL
• Apperance: Granular; powder; solid
• Density: 1.064 g/mL at 20 °C
• Vapor Density: 4.9 (vs air)
• Absorption Wavelength: A1M/260, H2O <0.05; A1M/280, H2O <0.05; λ: 220 nm Amax: 0.1; λ: 230 nm Amax: 0.05; λ: 240 nm Amax: 0.04; λ: 250 nm Amax: 0.03; λ: 260 nm Amax: ≤0.025; λ: 260 nm Amax: 0.019; λ: 260 nm Amax: 0.02; λ: 280 nm Amax: ≤0.020; λ: 280 nm Amax: 0.016; λ: 500 nm Amax: 0.02; λ: 500 nm Amax: 0.03
• pKa: (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid); (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid) (25°C)
• pH: 8.0-11.0 (20 °C, 1 M in H2O); 8.4-9.6 (20 °C, 1%); 8.7-9.3 (25 °C, 5% in solution); 8.7-9.3 (25 °C, 50 mg/mL in H2O); 8.8-9.4 (25 °C, 0.5 M in H2O); 8.8-9.4 (25 °C, 50 mg/mL in H2O); 8.9-9.2 (20 °C, 5%)

Safety Information

• Storage Warning: Hygroscopic
• RTECS: WC4500000
• European Hazard Symbols: Irritant (Xi)
• German water hazard class: 1; 3; nwg
• Risk Statements: 36/37/38
• Safety Statements: 26; 26-37-60
• TSCA Listed: 是
• GHS Pictograms: GHS07
• GHS Hazard statements: H315-H319-H335
• GHS Precautionary statements: P261-P305+P351+P338-P302+P352-P321-P405-P501A
• Personal Protective Equipment: Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Gloves; Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Regulation Compliance: FDA 21 CFR (175.210); FDA 21 CFR (175.300); FDA 21 CFR (182.1778); FDA 21 CFR (182.6290)

Product Information

• Purity: ≥98%; ≥98.0% (T); ≥98.5% (titration); ≥99%; ≥99.0%; ≥99.0% (T); ≥99.5%; ≥99.5% (T); ≥99.999% (metals basis); 98+%; 98-100.5% (calc. to the dried substance); 99.0% min; 99.95% trace metals basis
• Concentration: 0.5 M in H2O; 1000 mg/kg PO43-; 1000 mg/L PO43- in H2O
• Grade: ACS reagent; for electrophoresis; for HPLC; for ion chromatography; for molecular biology; for trace analysis; JIS special grade; Ph Eur; puriss.; puriss. p.a.; purum p.a.; ReagentPlus®; SAJ first grade; TraceCERT®; TraceSELECT®
• Suitability: complies for appearance of solution; corresponds to standard for filter test; corresponds to standard for RP gradient test; suitable for cell culture; suitable for electrophoresis buffers for the Weber and Osborn system; suitable for insect cell culture
• Impurities: ≤0.001% heavy metals (as Pb); ≤0.001% total nitrogen (N); ≤0.002% ammonium (NH4); ≤0.002% Chloride (Cl); ≤0.01% Insoluble matter; ≤0.01% insolubles; ≤0.1% Insoluble matter; ≤0.2% water-insoluble matter; ≤1% NaH2PO4(calc. to dried subst.); ≤10 mg/kg total nitrogen (N); ≤2.0% pyrophosphate (as Na4P2O7); DNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; reducing matter, complies; residual solvents, complies; RNases, none detected
• Cation Traces: Al: ≤0.0005%; Al: ≤1 mg/kg; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; As: ≤0.2 mg/kg; As: ≤1 mg/kg; As: ≤2 mg/kg; Ba: ≤1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤1 mg/kg; Bi: ≤5 mg/kg; Ca: ≤0.002%; Ca: ≤0.5 mg/kg; Ca: ≤10 mg/kg; Ca: ≤5 mg/kg; Ca: ≤50 mg/kg; Cd: ≤0.01 mg/kg; Cd: ≤1 mg/kg; Cd: ≤5 mg/kg; Cd: ≤50 mg/kg; Ce: ≤0.01 mg/kg; Co: ≤0.01 mg/kg; Co: ≤1 mg/kg; Co: ≤5 mg/kg; Co: ≤50 mg/kg; Cr: ≤1 mg/kg; Cr: ≤5 mg/kg; Cs: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤0.01 mg/kg; Cu: ≤1 mg/kg; Cu: ≤10 mg/kg; Cu: ≤5 mg/kg; Cu: ≤50 mg/kg; Eu: ≤0.01 mg/kg; Fe: ≤0.002%; Fe: ≤0.05 mg/kg; Fe: ≤1 mg/kg; Fe: ≤20 mg/kg; Fe: ≤20 ppm; Fe: ≤5 mg/kg; Fe: ≤50 mg/kg; heavy metals (as Pb): ≤0.001%; heavy metals (as Pb): ≤10 ppm; heavy metals: ≤0.001%; Hg: ≤0.005 mg/kg; Hg: ≤1 mg/kg; K: ≤0.005%; K: ≤100 mg/kg; K: ≤2 mg/kg; K: ≤20 mg/kg; K: ≤200 mg/kg; K: ≤50 mg/kg; La: ≤0.01 mg/kg; Li: ≤1 mg/kg; Li: ≤5 mg/kg; Mg: ≤0.005%; Mg: ≤0.1 mg/kg; Mg: ≤1 mg/kg; Mg: ≤5 mg/kg; Mn: ≤0.01 mg/kg; Mn: ≤1 mg/kg; Mn: ≤5 mg/kg; Mo: ≤1 mg/kg; Mo: ≤5 mg/kg; NH4+: ≤0.05%; Ni: ≤0.01 mg/kg; Ni: ≤1 mg/kg; Ni: ≤5 mg/kg; Ni: ≤50 mg/kg; Pb: ≤0.001%; Pb: ≤0.01 mg/kg; Pb: ≤2 mg/kg; Pb: ≤4 mg/kg; Pb: ≤5 mg/kg; Pb: ≤50 mg/kg; Rb: ≤5 mg/kg; Sm: ≤0.01 mg/kg; Sr: ≤0.5 mg/kg; Sr: ≤1 mg/kg; Sr: ≤5 mg/kg; Tl: ≤0.01 mg/kg; Y: ≤0.01 mg/kg; Yb: ≤0.01 mg/kg; Zn: ≤0.0005%; Zn: ≤0.01 mg/kg; Zn: ≤1 mg/kg; Zn: ≤10 mg/kg; Zn: ≤5 mg/kg; Zn: ≤50 mg/kg
• Antion Traces: chloride (Cl-): ≤0.002%; chloride (Cl-): ≤0.05%; chloride (Cl-): ≤10 mg/kg; chloride (Cl-): ≤40 ppm; chloride (Cl-): ≤5 mg/kg; chloride (Cl-): ≤50 mg/kg; fluoride (F-): ≤10 mg/kg; sulfate (SO42-): ≤0.005%; sulfate (SO42-): ≤0.05%; sulfate (SO42-): ≤1000 mg/kg; sulfate (SO42-): ≤200 mg/kg; sulfate (SO42-): ≤30 mg/kg; sulfate (SO42-): ≤50 mg/kg
• Foreign Activity: DNase, RNase and protease, none detected
• Loss on Drying: ≤0.1% loss on drying, 110 °C; ≤0.2% loss on drying; ≤0.2% loss on drying, 105 °C; ≤0.2% loss on drying, 105 °C, 2 h; ≤0.2% loss on drying, 105°C; ≤1% loss on drying, 105°C, 4h; ≤1% loss on drying, 130 °C; ≤2% loss on drying, 110 °C
• pH Range: 8.7 - 9.3
• Quality Level: PREMIUM
• Quality: ampule; certified by BAM, according to ISO 17025; meets analytical specification of Ph. Eur., BP, USP, FCC, E 339
• λ: 0.5 M in H2O; in concentrate
• Pharmacopeia: testing & handling conforms to Pharmacopeia
• Product Line: BioUltra
• Linear Formula: Na2HPO4

DETAILS

Sigma Aldrich - W239901

Packaging
1 kg in poly bottle
1 sample in glass bottle
5 kg in poly drum

Sigma Aldrich - 219886

Application
Suitable for buffer solutions

Sigma Aldrich - 71639

Other Notes
Primary standard buffer1

Sigma Aldrich - 81193

Analysis Note
Exact concentration, expiry date, traceability and detailed information on certification can be found on the certificate and certification report, included in each package. Content and expiry date on label.
Preparation Note
prepared with high purity Na2HPO4 and high purity water (18 MOhm, filtered 0.22 μm)

Sigma Aldrich - 71648

Packaging
concentrate (~0.33 M) in ampules for 6x1 l 0.005 M ready-to-use solution
Reconstitution
recommendation for use in ion pair chromatography

Sigma Aldrich - 38364

Analysis Note
This certified reference material (CRM) is produced and certified in accordance with ISO/IEC 17025 and ISO Guide 34. This CRM is traceable to SI unit kg and measured against NIST SRM. Certified content incl. uncertainty and expiry date are given on label. Download your certificate at: http://www.sigma-aldrich.com.
General description
1000 mg/L PO4-3 in water, prepared with high purity Na2HPO4 and water TraceSELECT®Ultra.
Legal Information
TraceCERT is a registered trademark of Sigma-Aldrich Co. LLC
TraceSELECT is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 255793

Packaging
10, 50 g in poly bottle

Sigma Aldrich - 71629

Legal Information
TraceSELECT is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 28-3770

Suitability
pH standard solution

Sigma Aldrich - S0876

包装
1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
法律信息
ReagentPlus 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - S9763

包装
1, 2.5 kg in poly bottle
100, 500 g in poly bottle
5, 12 kg in poly drum

Sigma Aldrich - 71651

Packaging
concentrate (~0.50 M) in ampules for 6x1 l 0.01 M ready-to-use solution
Reconstitution
recommendation for use in ion pair chromatography